摘要
用重量法和柱层析法从苦茶Ｃａｍｅｌｌｉａ ａｓｓａｍｉｃａ ｖａｒｋｕｃｈａ Ｃｈａｎｇ ｅｔ Ｗａｎｇ 的嫩梢中分离出３ 种嘌呤生物碱， 经薄层层析、熔点测定、元素分析、核磁共振和质谱等对其结构的测定，鉴定为咖啡碱、１ ， ３ ， ７ ， ９四甲基尿酸和可可碱， 其百分含量分别为０８８％ ， ０８４ ％ 和０２９ ％ ． 样品水提物经氯仿萃取后直接由高压液相色谱分析， 测得苦茶含咖啡碱１９３２ ９％ ，四甲基尿酸１２９４ １ ％ ， 可可碱０５８５ ５ ％ ， 茶叶碱００１２ ８％ ．
Purine alkaloids in Camellia assamica var. Kucha Chang et Wang were investigated by extraction combined with silica gel column chromatography. Three products were isolated. Product A and C were determined as caffeine and theobromine by TLC and melting point compared with standards. The structure of product B were determined by MS and 1HNMR, the element analysis and melting point for 1,3,7,9 tetramethyluric acid.The purine alkaoids were consisted of caffeine, 1,3,7,9 tetramethyluric acid, theobromine and theophylline in the leaves of C.assamica var. kucha , and their content was 1 932 9%, 1 294 1%, 0 585 5% and 0 012 8% respectively by HPLC.The distribution of new type of purine alkaloids and such high content of tetramethyluric acid in wild tea tree resources is firstly reported.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期82-86,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金
关键词
苦茶
嘌呤生物碱
咖啡碱
山茶属
camellia assamica var
kucha
dominant pruine alkaloid
1
3
7
9 tetramethyluric acid
caffeine
