摘要
目的：探讨人参皂甙Rg3（GS-Rg3）对兔耳增生性瘢痕形成的影响，为其抑制瘢痕增生研究提供实验依据。方法：大耳白兔24只，切除2cm×2cm全层皮肤，每耳4～6处，建立瘢痕动物模型，左右耳自身对照。实验组每3d局部注射1次GS-Rg30．1mL（浓度3g·L^-1），对照组注射等体积的生理盐水。分别于用药后2、4及6周切取瘢痕组织，肉眼观察瘢痕组织的厚度，显微镜下观察组织形态学变化，检测增殖细胞核抗原（PCNA）、Bcl-2及Bax的表达情况。结果：对照组兔耳创面上皮化后3周时，增生厚度为兔耳腹侧皮肤全层厚度的3～4倍，镜下可见真皮组织明显增生、变厚，由大量成纤维细胞、胶原组织及血管组成，胶原排列不整齐，呈结节或旋涡状分布，部分可见对应处软骨细胞增生。实验组6周时皮肤变薄，胶原排列较整齐，成纤维细胞及血管数量减少。增生性瘢痕组织中有大量PCNA及Bcl～2蛋白质的表达，阳性表达率分别为（39．55±6．07）％和（56．92±10．56）％，明显高于对照侧正常皮肤组织的阳性表达率（11．18％±1．71％和12．12±2．87％）（P〈0．01）。实验组在注射GS-Rg32周后Bcl-2蛋白质表达量逐渐下降，6周时明显减少，与注射前比较差异有显著性（P〈0．01）；注射GS-Rg32周后Bax表达量明显增加，与注射前比较差异有显著性（P〈0．01）。结论：GS-Rg3对兔耳增生性瘢痕有抑制作用，可使瘢痕组织的纤维化程度明显减轻。
ears and Objective provide To investigate the effects of ginsenoside Rg3 （GS-Rg3） on experimental foundation for study on its inhibition the hypertrophic scars of rabbit on the hypertrophic scars. Methods Hypertrophic scars were proved on 24 white rabbits, of which the whole level of the skin was excised for 2 cm×2 cm, 4-6 points for each ear, controlled by itself. GS-Rg3 0.1 mL （concentration 3 g · L^-1 ） was injected into experimental group and the same volume of saline solution into control group, once every three days regionally. The scar tissues were collected 2, 4 and 6 weeks after the injection respectively, the thickness of the scar, structure under the microscope, and the expressions of PCNA, Bcl-2 and Bax were observed. Results In control group, three weeks after the epithelization of the wound, the thickness of the hypertrophic tissue was 3-4 times of ventro ear skin. Under microscope, the dermis was hyperplasia and got thicker, consisted with amount of fibroblast cells, collagen and vessels, the collagen was untidy, nodule or vortex, and the cartilage could beobserved in some region. In experimental group, six weeks after the injection, the skin got thinner, the collagen became neath and the quantity of the vessels decreased. In the hypertrophic scars, there was high expression of PCNA, the percent of positive cells was higher （39.55 ± 6.07 %） compared normal tissue （11.18± 1.71%）. In GS-Rg3 group, the expression of Bcl-2 was gradually decreased two weeks after injection and obviously decreased six weeks later, there was significant difference compared with before injection （P〈 0.01）, and that of Bax increased significantly two weeks after injection compared with before injection （P〈0.01）. Conclusion GS-Rg3 can inhibit the hypertrophic scars in rabbit ear and decrease the fibrosis degree of the scar tissue.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第4期628-632,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(20050413-1)
关键词
人参皂苷
瘢痕
疾病模型
动物
ginsenoside
cicatrix
disease models, animal
