摘要
目的建立不同损伤程度的创伤性脑损伤模型。方法利用Weiss Young改良的重物打击装置,对36只Wistar大鼠右侧大脑分别给予20g·cm、50g·cm和100g·cm的打击强度致伤。观察大鼠在损伤后不同时间的全身反应、皮层和海马组织的病理学变化及大鼠行为学改变。结果20g·cm打击组大鼠全身反应无异常,皮质轻微挫伤,大鼠逃避反应与伤前无显著差异(P>0.05);50g·cm打击组大鼠全身反应轻度异常,皮质挫裂伤,而海马结构基本正常,大鼠逃避反应潜伏期延长(P<0.05);100g·cm打击组大鼠全身反应严重异常,皮质及海马均发生明显挫裂伤,大鼠逃避反应潜伏期明显长于前两组(P<0.05)。结论利用Weiss Young改良的重物打击装置,采用20g·cm、50g·cm和100g·cm的打击强度可较好地制备轻度、中度和重度脑创伤模型。
Objective To establish traumatic brain injury （TBI） model with different severity in degree, Methods The right cerebra in 30 Wistar rats, who were averagely into 3 groups, were injured respective by 20 g·cm, 50 g·cm and 100 g·cm impact strength released from the weight drop equipment improved by Weiss Yong. The changes in the systemic reaction, pathology of cortexes and hippocampi, and the behavior were observed after TBI in all the rats. Results The only mild contusion of cortexes was produced by 20 g·cm strength. The middle contusion of cortex was observed and the hippocampal structure is normal in 50 g·cm strength group. The strength of 100 g·cm led to significant contusion of cortexes and hippocampi. The latency of avoidance reaction in 20 g·cm group was significantly longer only 1 day after TBI than that in the control group （P〈0.01）. The latency of avoidance reaction in 50 g·cm group was significantly longer 1, 3 and 7 days after TBI than those in 20 g·cm group and control group （P〈0.05 or P〈0.01）. The latency of avoidance reaction in 100 g·cm group was significant longer than those in the other 3 groups （P〈0.05 or P〈0.01）. Conclusions The traumatic brain injury models with mild, moderate and severe brain injury can be well established by 20 g·cm, 50 g· cm and 100 g·cm impact strength released from weight drop equipment improved by Weiss Young.
出处
《中国临床神经外科杂志》
2008年第7期416-419,共4页
Chinese Journal of Clinical Neurosurgery
基金
国家海外青年学者基金资助(30728026)
关键词
创伤性脑损伤
动物模型
建立
Traumatic brain injury
Model
Establishment
Rats
