摘要
用NADPH脱氢酶组化及Parvalbumin免疫组化双标记技术观察了正常大鼠视网膜一氧化氮合酶(NOS)与Parvalbumin(PV)的分布,结果显示NOS阳性神经元主要位于内核层内缘带第二列,少数位于节细胞层,胞体圆形/卵圆形,直径8～12μm,细胞一侧发出突起伸向内网层1、3、5亚层,以第3亚层最为明显,PV免疫反应(PV—Ⅰ)神经元位于内核层最内缘第一列,少数位于第二列、中间部及节细胞层,胞体卵圆形,直径6～10μm,由胞体一端发出突起伸向内网层第1、5亚层.神经纤维层可见PV～Ⅰ纤维.内核层内缘第二列可见少数双标阳性细胞,在它们的PV免疫反应胞质内散布有NOS颗粒.实验结果表明 NOS阳性神经元与PV～Ⅰ神经元均为无长突细胞,分属不同的亚型,少效PV～Ⅰ神经元属节细胞,个别双标细胞可能为另一种亚型的无长突细胞,提示NOS与PV在视觉信息传递中可能存在某些联系.
The distribution of NOS and Parvalbumin (PV) in Rat's Retina was Observed by the double staining technique of NADPH-diaphorase histochemical and PV immunohistochemical method The results showed that NOS positive nurons mainly located in the second row of inner nuclear layer (INL). The diameter of the cells were from 8?m to 12μm. Their processes extended toward inner plexiform layer (IPL) and located in 1st、3rd、5th sublamina, especially 3rd sublamina. The PV immunoreactive (PV～Ⅰ) neurons mainly distributed in the innermost row of INL, the other were in the second row and the middle of INL, and the ganglion cell layer (GCL). The diameter of PV～Ⅰ neurons were from 6μm to 10μm Their processes extend toward IPL and located in 1st、5th sublamina. In the second row of INL there were a little double staining neurons. The results suggested that NOS positive neurons and PV～Ⅰ neurons were different subtype of amacrine cells. Some PV～Ⅰ neurons belonged in ganglion cells. The double staining cells may be another subtype of amacrine cells. It was indicated that NOS and PV maintained close relations in the vision transfer.
