摘要
选用1日龄天府肉鸭360只，随机分为6组，分别以对照日粮（Cu8mg／kg）和高铜日粮（Ⅰ组：Cu100mg／kg，Ⅱ组：Cu200mg／kg，Ⅲ组：Cu400mg／kg，Ⅳ组：Cu600mg／kg，Ⅴ组：Cu800mg／kg）饲喂6周，对高铜引起的雏鸭脾的病理损伤进行了观察。结果显示，Ⅰ、Ⅱ组雏鸭的脾组织学变化与对照组比较差别不明显，Ⅲ、Ⅳ、Ⅴ组脾出现不同程度的淋巴细胞减少，电镜下，Ⅲ、Ⅳ、Ⅴ组脾淋巴细胞线粒体肿大，嵴断裂甚至消失，核染色质积聚于核膜下呈环形或团块状；Ⅲ、Ⅳ、Ⅴ组脾的绝对重量、生长指数显著降低（P〈0．05或P〈0．01）；Ⅲ、Ⅳ、Ⅴ组脾淋巴细胞的静止期延长，增殖指数明显下降（P〈0．05或P〈0．01）；Ⅲ、Ⅳ、Ⅴ组脾淋巴细胞的凋亡率显著高于对照组（P〈0．05或P〈0．01）。证实，日粮铜含量400～800mg／kg可不同程度地抑制雏鸭脾的发育，导致雏鸭细胞免疫功能和体液免疫功能受损。
To observe the pathological lesions caused by high copper on the duckling spleen,360 oneday-old meat-type Tianfu ducklings were divided randomly into control,and Groups Ⅰ ,Ⅱ , Ⅲ, Ⅳ and Ⅴ , which were fed with diets supplemented with 8,100,200,400,600 and 800 mg Cu per kilogram for 6 weeks,respectively. Histological changes were observed in ducklings in Groups Ⅲ , Ⅳ and Ⅴ in comparison with those in the control. Ultrastructurally,the mitochondria in these three groups were swelled,and their crista were broken and disappeared. The chromatins in Groups Ⅲ ,Ⅳ and Ⅴ were also condensed. The weight and the proliferating index of spleen were lower in Groups Ⅲ,Ⅳand Ⅴ than those in the control group（P〈0.05 or P 〈0.01）. The number of G0＋ G1 phases splenoeytes was high, and the proliferating index was low in Groups Ⅲ,Ⅳ and Ⅴ compared with those in the control group（P〈0. 05 or P〈0. 01）. Percentages of apoptotie lymphoeytes in spleen were higher in Groups Ⅲ, Ⅳ and Ⅴ than that in the control group（P〈0. 05 or P〈0. 01）. The results showed that the contents of copper in diet from 400 mg/kg to 800 mg/kg could inhibit the development of spleen,cause pathological lesions in the spleen and impair cellular and humoral immune function of the ducklings.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第7期616-621,共6页
Chinese Veterinary Science
基金
国家自然科学基金项目(30471304)
四川省教育厅重点项目(2002A002)
四川省科技厅重点项目(03JY029-030-2)
关键词
高铜
脾
病理变化
生长指数
细胞周期
雏鸭
high eopper
spleen
lesion
proliferating index
cell cycle
duekling
