摘要
建立高效液相色谱法测定盐酸尼卡地平缓释片的含量。采用高效液相色谱法，色谱柱为Waters XTerra^TM RP18柱；以甲醇-0．01mol·L^-1磷酸二氢钾溶液（72：28）为流动相，流速为1．0mL·min^-1；检测波长为236nm；柱温为35℃。浓度在5．008-95．152μg·mL^-1（r=0.9999）范围内具有良好线性关系，平均回收率为99．28％，RSD为0．46％（n=9）。方法可靠、简单可行，可用于测定盐酸尼卡地平缓释片的含量。
A method for determination of Nicardipine Hydrochloride Sustained--release Tablets was developed. HPLC method was carried out on a Waters XTerraTM RP18 column with UV detection at 236nm. The mobile phase was methanol-0. 01mol · L^-1 potassium dihydrogen phosphate （72 ： 28） with a flow rate of 1.0 mL · min^-1 and the column temperture was 35℃. The content of nicardipine hydrochloride was calculated by external standardization method. There was a good linear relationship for nicardipine hydrochloride within the range of 5. 008 - 95. 152μg · mL^-1 （r = 0. 9999）. The average recovery was 99. 28% and RSD was 0. 46% （n=9）. The method is simple, specific and not interfered by excipients. It is accurate for quality control of Nicardipine Hydrochloride Sustained-release Tablets.
出处
《中国药事》
CAS
2008年第7期576-577,共2页
Chinese Pharmaceutical Affairs
关键词
盐酸尼卡地平缓释片
HPLC
含量测定
Nicardipine Hydrochloride Sustained-release Tablets
HPLC
determination
