摘要
目的通过对宫颈病灶组织的人乳头瘤病毒分型基因芯片法和宫颈分泌物的PCR荧光定量法检测结果比较,为临床人乳头瘤病毒的诊断提供比较恰当的选择方法。方法采用人乳头瘤病毒分型基因芯片检测系统,对200例患者的子宫颈癌新鲜冻存组织进行人乳头瘤病毒分型检测,其中的134例患者同时采用荧光定量PCR方法对宫颈病灶分泌物进行人乳头瘤病毒16、18型检测。结果人乳头瘤病毒分型基因芯片检测的人乳头瘤病毒阳性率为94.00%(188/200),均为高危型。其中存在人乳头瘤病毒16和/或18的占90.00%(180/200),而荧光定量PCR检测的人乳头瘤病毒16和/或18阳性率为36.57%(49/134)。荧光定量法检测为阳性的49例均在基因芯片法检测结果中显示含有HPV16和/或18型。两种方法的人乳头瘤病毒检出率有明显差异(2χ=106.2862,P<0.005)。结论虽同以PCR技术为基础,但人乳头瘤病毒分型基因芯片检测系统的敏感性要优于荧光定量PCR检测方法,同时前者还可对人乳头瘤病毒分型,是临床应用的较好选择。
Objective To select an appropriate detecting method for diagnosis of HPV infection in clinic. Methods 200 fresh freezen tissues of cervical cancer were detected for HPV typing by using HPV genotyping chip system, of which, 134 of them were detected for HPV 16 and HPV 18 in secretion of cervical lesion by using fluorescence quantitative PCR method. Results The HPV positive rate by HPV genotyping chip system was 94.00% （188/200）, and all of them were high-risk types of HPV, of them, HPV16 or/and HPV18 accounted for 90.00% （180/200）. The HPV16 or / and HPV18 positive rate by fluorescence quantitative PCR method was 36.57% （49/134）. 49 cases of cervical tissues with positive HPV detected by using fluorescence quantitative PCR method contained HPV 16 and or HPV 18 by using HPV genotyping chip system. In HPV positive rate detected by the two detecting methods there was a significant difference （χ^2 = 106.2862, P 〈 0. 005 ）. Conclusion Although the two methods are both based on PCR technology, the sensitivity of HPV genotyping chip system is higher than that of fluorescence quantitative PCR method, meanwhile, the system can genotype HPV. So, it is better choice for clinical application.
出处
《中国妇幼健康研究》
2008年第4期342-343,共2页
Chinese Journal of Woman and Child Health Research
基金
海南省自然科学基金指导资助项目(80463)
关键词
人乳头瘤病毒
基因芯片法
荧光定量法
敏感性
HPV
HPV genotyping chip system
PCR fluorescence quantitative
sensitivity
