摘要
为探讨癌基因在原发性喉癌中的增殖调控作用，采用免疫组化ＳＡＢＣ方法对３６例原发性喉癌行Ｐ１６、Ｒｂ和周期蛋白Ｄ１（ＣｙｃｌｉｎＤ１）的检测，结果：Ｐ１６在肿瘤中阳性率３０．６％与癌旁组织阳性率６１．１％之间差异有显著性（Ｐ＜０．０５），Ｒｂ、ＣｙｃｌｉｎＤ１阳性率分别为６１．１％和４７．２％。Ｐ１６与Ｒｂ之间阳性率差异有显著性（Ｐ＜０．０５），多数情况下当Ｐ１６阳性时Ｒｂ阴性，反之亦然。经秩相关检验Ｒｂ与ＣｙｃｌｉｎＤ１阳性呈正相关。Ｐ１６、Ｒｂ在肿瘤高、低分化之间表达差异有显著性（Ｐ＜０．０５）。同一抗体在不同的临床分期及肿瘤部位和同一分期及部位在不同的抗体中表达差异不显著。结果显示：由Ｐ１６、Ｒｂ及ＣｙｃｌｉｎＤ１等形成的反馈调节链在喉癌的增殖调控中起重要作用。
To determine the effect of gene of carcinorma on the proliferation and regulation in primary laryngeal cancer, P16, Rb, Cyclin D1 were examined with immunohistochemical SABC method in 36 cases of primary laryngeal cancer. The results showed: P16 was positive in 11 of 36 in carcinoma specimens (30.6%) , 22 of 36 in the specimens surrounding the carcinoma (61.1%) were positive. The difference was significant ( P <0.05).The positive rates of Rb and Cyclin D1 were 61.1% and 47.2% respectively. Comparing the positive rate of P16 to Rb was more significantly ( P <0. 05). In most circumstances, when Rb was positive , P16 was negative and vice versa. The positive rate of Rb correlated with the one of Cyclin D1 positively by rank correlation. The expression of P16 and Rb was significantly different between well and poor tissues differenciation. The expression had no significant difference in the same antibody in various clinical stage and position or in the same stage and position but different antibody . The results suggest that the feedback regulation chain consisted of P16 plays an important role in the proliferation and regulation.
出处
《中华耳鼻咽喉科杂志》
CSCD
1997年第5期299-301,共3页
Chinese Journal of Otorhinolaryngology
