摘要
目的:研究喉声门上型鳞癌中血管内皮生长因子(vascularendothelialgrowthfactor,VEGF),p16和CD44v6的定量表达与颈淋巴结转移的关系。方法:应用S-P免疫组化染色方法检测喉声门上型鳞癌、慢性炎症、不典型增生等组织中的VEGF、p16和CD44v6表达,用彩色病理图像分析系统对切片的免疫组化染色结果进行定量测量。结果:22例喉部非癌疾病组与76例喉声门上型鳞癌组相比较,两组VEGF、p16和CD44v6的定量表达水平均有显著性差异(P<0.01)。喉声门上型鳞癌无颈淋巴结转移组28例与颈淋巴结转移组48例相比较,VEGF和p16的定量表达水平有显著性差异(P<0.01),但CD44v6的定量表达水平无显著性差异(P>0.05)。结论:VEGF的高表达、p16的低表达与喉声门上型鳞癌细胞的发生和转移呈相关性。VEGF和p16的定量表达对于喉声门上型鳞癌颈淋巴结转移的预后分析是一个有价值的参考指标。
Objective: The aim of this study was to investigate the relationship between the quantitative expressions of vascular endothelial growth factor(VEGF), p16, and CD44v6 in supraglottic squamous cell carcinoma (SGSCC) and the cervical lymph node metastatic status. Methods: Seventy six cases of SGSCC (28 N+,26 N0,22 cN0-pN+)and twenty two cases of non neoplastic laryngeal diseases were immunostained by streptavidin peroxidase method. Quantitative expressions of VEGF, p16, and CD44v6 genes in the sections were determined by the Colorful Multiple Image Analysis System. Results: The significantly statistic differences exist between the quantitative expressions of VEGF,p16,and CD44v6 in SGSCC and those in the non neoplastic laryngeal diseases (P< 0.01). Significantly statistical differences exist between the quantitative expressions of VEGF, p16 in the cervical lymph node metastatic group and in no lymph node metastatic group (P< 0.01). There is no significantly statistical difference of the CD44v6 quantitative expression between lymph node metastatic group and in the no lymph node metastatic group (P >0.05). Conclusions: The quantitative expression of VEGF and p16 is correlated with the growth, metastasis of carcinoma cells in SGSCC, they may be valuable reference to predict the cervical metastatic status in SGSCC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第9期970-973,共4页
Chinese Journal of Cancer
