摘要
目的：研究吉林省长春市0～12周岁儿童野生型麻疹病毒的基因型别及核蛋白（N）基因序列特征,探讨控制麻疹发生的措施。方法：采用Vero/SLAM细胞分离培养麻疹病毒,通过逆转录-聚合酶链反应（RT-PCR）方法从分离的麻疹病毒中扩增核蛋白（N）基因羧基端450个核苷酸片段（bp）并进行核苷酸测序分析,对基因库中麻疹病毒的22个代表株基因序列的同源性进行比较。结果：分离得到9株野生型麻疹病毒,N基因与H1基因型代表株相比同源性为98.3%。结论：吉林省长春市麻疹流行株属于H1基因型。
Objective： To study the genetic characteristics and genotype of measles strains and the measures to control measles in Jilin province. Methods： Throat swabs were collected for virus isolation with Vero/SLAM cells. 450 nucleotides fragment of the C - terminal of the nucleoprotein （N） gene were amplified by RT - PCR and subjected to sequence and phylogenetic analysis using DNAstar software. Results： Nine strains were isolated, N genes homogeneity was 98.3% compared with other wild - type viruses of H1 genotype. Conclusion： The circulating measles viruses are belong to clade H1 in Jilin province.
出处
《中国妇幼保健》
CAS
北大核心
2008年第10期1407-1409,共3页
Maternal and Child Health Care of China
关键词
麻疹病毒
核蛋白N
序列分析
Measles virus
Nucleoprotein gene
Sequence analysis
