摘要
目的 2015年3-5月调查吉林省长春市和吉林市肉鸽与信鸽中鹦鹉热衣原体的流行情况及基因型分布。方法本研究共采集鸽子粪便样本399份,其中长春市样本282份,吉林市样本117份。利用PCR技术进行鹦鹉热衣原体ompA基因扩增、测序以及基因型分析。结果本实验结果显示:鹦鹉热衣原体的感染率为5.01%(21/399),其中吉林市鹦鹉热衣原体的感染率(9.40%)明显高于长春市的感染率(3.19%)。此外,品种也是与衣原体感染相关的主要风险因素,肉鸽的感染率为7.49%,而信鸽的感染率为0。ompA基因的序列分析显示,这些鹦鹉热衣原体都属于B型。结论综上所述,我国吉林省肉鸽具有较高的B型鹦鹉热衣原体流行,给人类的健康带来了潜在的威胁。
Chlamydia psittaci is a causative agent of psittacosis,which can infect a wide range of hosts including birds and humans.However,information regarding C.psittaci infection in pigeons is scarce.In the present study,a total of 399 fecal samples from pigeons were collected from Jilin Province,northeastern China,between March and May 2015,and examined by nested PCR amplification of outer membrane protein A（ompA）gene.The overall Chlamydiosis prevalence was 5.01%（21/399）,with 3.19%in Changchun City and 9.40%in Jilin City.Furthermore,breed was the major risk factor associated with Chlamydiainfection in pigeon,boiler pigeons had a prevalence of 7.49%,whereas no C.psittaci was detected in racing pigeons.Sequence analysis of the ompAgene revealed that all the identified isolates represented C.psittaci genotype B.Our results firstly indicated the presence of zoonotic C.psittaci in boiler pigeons in Jilin Province,northeastern China,and effective measures should be implemented to reduce the risk of C.psittaci transmission from pigeons to humans.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2017年第2期104-109,共6页
Chinese Journal of Zoonoses
基金
Supported by the China Agricultural Science and Technology Innovation Program(ASTIP)(No.CAAS-ASTIP-2014-LVRI-03)
the Fundamental Research Funds of Chinese Academy of Agricultural Sciences(No.Y2016JC05)~~
关键词
鹦鹉热衣原体
鸽子
基因型
吉林省
Chlamydia psittaci
pigeon
genotypes
Jilin Province
