摘要
以岩原鲤(Procypris rabaudi Tchang)肠道的粗酶液作为酶源,采用离体消化法测定鱼粉、豆粕、菜粕、棉粕、膨化豆粕、膨化菜粕、膨化棉粕等7种蛋白饲料的干物质和蛋白质的离体消化率及其4h内氨基酸生成量随反应时间的关系和氨基酸的生成速度。结果表明:(1)岩原鲤对鱼粉干物质的消化率和酶解氨基酸的生成速度均为最高,其次为豆粕;以菜粕、棉粕的效果最差。其中,鱼粉、豆粕、菜粕、棉粕的干物质消化率分别为47.95%、40.46%、28.16%、16.74%,酶解氨基酸的生成速度分别为21.800mg·h-1、17.233mg·h-1、13.033mg·h-1、14.100mg·h-1。同时,肠道酶液对测定饲料的蛋白质离体消化率均大于肝胰脏酶液。(2)棉粕和菜粕膨化后,岩原鲤对干物质、蛋白质的离体消化率以及酶解氨基酸的生成速度都增加。其中,棉粕膨化后干物质、蛋白质的离体消化率在肠道分别增加6.25%、10.53%,在肝胰脏分别增加8.43%、8.15%;菜粕膨化后干物质、蛋白质的离体消化率在肠道分别增加9.30%、7.72%,在肝胰脏分别增加7.99%、11.20%;棉粕和菜粕膨化后的酶解氨基酸的生成速度分别增加10.74%、16.08%。而豆粕膨化后,其蛋白质的离体消化率以及酶解氨基酸的生成速度却显著下降。结论认为,岩原鲤对所用的7种人工配合饲料有较强的消化能力。
Procypris rabaudi was an endemic fish in upper reaches of the Yangtze River in china. In order to investigate the digestibile capacity for 7 protein feedstuffs, digest enzymes for the enzymolysis cultivation were extracted from intestine and hepatopancreas respectively. The apparent digestibilities of dry matter and crude protein for Procypris rabaudi to fish meal, soybean meal, rapeseed meal, cottonseed meal, extruded soybean meal, extruded rapeseed meal and extruded cottonseed meal were determined in vitro. The amino acid produced from the enzymolysis fluid of seven proteins were measured by the method of ninhydrin in 0-4 hour. The regression equations of the amino acid to time were conducted respectively. The amino acid production rate from 7 proteins were measured. The results showed that： （1） The digestibility of dry matter, crude protein and the enzymolysis rates of fish meal was higher than that of soybean meal, rapeseed meal or cottonseed meal, while the digestibility of rapeseed meal and cottonseed meal was the lowest. The digestibility of dry matter of fish meal, sotbean meal, rapeseed meal and cottonseed meal was 47.95%, 40.46%, 28.16%, 16.74%, respectively. and the enzymolysis rate of amino acid was 21.800 mg·h^-1, 17.233 mg·h^-1, 13.033 mg·h^-1, 14.100mg·h^-1, respectively. （2） Compared to extruded soybean meal, rapeseed meal and cottonseed meal, the digestibility of dry matter, crude protein and the enzymolysis rates of extruded rapeseed meal and extruded cottonseed meal significantly increased. The digesitibility of dry matter and protein for extruded cottonseed meal in vitro were increased by 6.25% and 10.53% in intestine, by 8.43% and 8.15% in hepatopancreas, and that of extruded rapeseed meal were increased by 9.30%and 7.72% in intestine, by 7.99% and 11.20% in hepatopancreas compared to non-extrused rapeseed meal.The enzymolysis rate of amino acid for extruded cottonseed meal and extrused rapeseed meal were increased by 10.74% and 16.08% compared to non-extruded one, respectively. However, the digestibility of extruded soybean meal significantly decreased. In conculusion, Procypris rabaudi has a good ability to digest 7 tested feedstuffs.
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第4期637-643,共7页
Journal of Fishery Sciences of China
基金
重庆市教委资助项目(001905)
关键词
岩原鲤
蛋白质饲料
消化率
离体研究
Procypris rabaudi
protein feedstuff
digestibility
in vitro study
