摘要
采用离体灌流孵育的方法研究了１７β－雌二醇（Ｅ２）对鲤鱼脑垂体生长激素（ＧＨ）分泌的影响．结果表明：１μｍｏｌ／ＬＥ２作用于性腺成熟期鲤鱼脑垂体碎片３０ｍｉｎ后，ＧＨ基础分泌显著提高，２ｈ后达到最大值，随后逐步下降，４ｈ后ＧＨ基础分泌水平与作用前无显著性差异．用Ｅ２（１μｍｏｌ／Ｌ）进行１０～１２ｈ的过夜孵育，ＧＨ的基础分泌无显著性变化，但影响了脑垂体对ＬＨＲＨ－Ａ和ＴＲＨ刺激的反应性，对前者有抑制作用。
The effect of 17βEstradiol on the growth hormone secretion by the pituitary of common carp was studied by using in vitro perifusion system for pituitary fragments. The basal GH release rose significantly 30 min after the introduction of E2(1 μmol/L) to the pituitary fragments of sexually mature common carp(GSI:15 6%~25 4%), and reached the highest value of (377 8±99 5) ng/mL after 2 h. Then it reduced to (188 6±65 2) ng/mL without significant difference to the value before the E2 was introducted after 240 min of the E2 treatment.In this study, overnight E2 treatment did not alter the basal GH release. But it indicated that the effects of prolonged E2 exposure on LHRHstimulated and TRHstimulated GH release in common carp can be exerted directly at the pituitary level. E2 played an inhibition role in the LHRHstimulated GH release but a promoting role in the TRHstimulated one.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期23-27,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省自然科学基金
关键词
17Β-雌二醇
生长激素
离体实验
鲤鱼
17βEstradiol,growth hormone,thyrotropinreleasing hormone, analog of gonadotropinreleasing hormone, perifusion,common carp
