摘要
采用人工土壤法研究了染毒14 d后不同浓度(0、10、20、40、80 mg.kg-1)异恶草酮对赤子爱胜蚓(Eisenia foetida)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,总抗氧化能力(T-AOC)和体腔细胞DNA损伤的影响。结果表明:随着暴露浓度的增加,SOD活性呈现先上升后逐渐下降的趋势,低浓度(10、20 mg.kg-1)能显著诱导SOD酶活性(P<0.01,P<0.05),诱导率分别为19.56%、13.03%。CAT活性在较低浓度时没有显著性变化,当浓度达到40、80 mg.kg-1时出现显著性诱导(P<0.01),诱导率分别为43.42%、68.08%。T-AOC在整个胁迫阶段呈现先降低后上升的趋势,最终趋于正常水平,20 mg.kg-1暴露组T-AOC含量最低(5.74 U.mg-1pro),比对照组减少了44.54%。GSH-Px酶活性在整个暴露阶段处于抑制状态。不同浓度的异恶草酮均能引起蚯蚓体腔细胞DNA损伤,暴露浓度与尾部DNA含量、Olive尾距和尾长具有良好的剂量-效应关系(P<0.01)。上述结果表明抗氧化酶活性及DNA损伤可作为异恶草酮影响的生物标志物,异恶草酮对土壤动物蚯蚓具有毒性作用,对土壤环境具有潜在的生态风险。
Effects of clomazone at different concentration(0 mg·kg-1,10 mg·kg-1,20 mg·kg-1,40 mg·kg-1,80 mg·kg-1) on activity of antioxidative enzymes of SOD,CAT,GSH-Px,T-AOC and DNA damage in earthworm(Eisenia foetida) were studied with an artificial soil test in 14 days of exposure.During the exposure period,there was a trend on the SOD activity by an initial significant increase(P<0.05) and then following a gradual decrease.When the earthworms were exposed to 10 mg·kg-1 and 20 mg·kg-1 of clomazone,the activity of SOD increased significantly with induced rate of 19.56% and 13.03%,respectively.The activity of CAT had no significant change at the low concentrations(10 mg·kg-1,20 mg·kg-1),and was induced at the higher concentrations(40 mg·kg-1,80 mg·kg-1) with induced rate of 43.42% and 68.08%,respectively.The content of T-AOC was inhibited first but induced,then recovering to the level of control gradually.The content of T-AOC exposed to the concentration of 20 mg·kg-1 decreased significantly with inhibition rate of 44.54%.The activity of GSH-Px was inhibited significantly during the period of exposure.The results showed that clomazone could induce DNA damage of earthworm coelomocytes at different exposure concentration.The significant dose-effect relationships were found among clomazone concentration and tail DNA percent,OTM as well as tail length(P<0.01).The activity of antioxidative enzymes and DNA damage could be as biomarkers to indicate the direct toxic effect of clomazone on soil animals.The results indicated that clomazone had a potential ecological risk on soil environment.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2013年第5期925-931,共7页
Journal of Agro-Environment Science
基金
农业部农药登记残留试验资助项目
关键词
蚯蚓
异恶草酮
抗氧化酶
DNA损伤
earthworm
clomazone
activity of antioxidative enzymes
DNA damage
