摘要
以南疆部分扁桃品种为试材,利用CTAB法提取基因组DNA,将PCR的主要成分设定4个梯度,优化了扁桃SSR技术中PCR反应体系,试验结果表明,适宜扁桃SSR技术体系为：在20μL反应体系中TaqDNA聚合酶和DNA最适用量分别为1U和100 ng;Mg^2＋、dNTP和引物最适终浓度分别为1.5 mmol/L、0.20 mmol/L和0.2μmol/L。利用18个扁桃品种验证此反应体系,非变性聚丙烯酰胺凝胶电泳检测结果显示,扩增产物大多在100-300 bp,多态性高,且反应体系的稳定性和可重复性好。
The partial varieties of almost in south of Xinjiang are used as the materials,the DNA was distilled by CTAB.Every factor of PCR had four different concentrations and its variation changed the result of PCR-SSR.The results showed that the optional SSR reaction system for almond was TaqE1U,100 ng,1.5 mmol/LMg^2＋,0.20 mmol/LdNTP,0.2 μmol/L primer in 20 μL.To retest the above SSR system,18 cultivars of almond were used.SSR franments between 100-300 bp were detected by polyacrylamide nel（8%）,indicatinn that the SSR reaction system was steady and reproducible.
出处
《西北农业学报》
CSCD
北大核心
2008年第3期219-222,241,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
兵团科技计划应用基础研究项目(2006jc04)
关键词
扁桃
SSR
优化
Almond,SSR,Optimization
