摘要
目的在基因转录水平了解12条细胞表面受体介导的信号通路相关基因对大鼠肝再生的调控作用。方法用搜集网站资料和查阅相关论文等方法获得上述基因,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用比较手术和假手术中基因的表达差异性确定肝再生相关基因。结果初步证实上述基因中491个与肝再生相关,其中226个基因之间有742种直接作用关系。细胞因子和趋化因子介导的酶联受体、G蛋白耦联受体、谷氨酸;抗原受体介导的,整合素介导的,脂多糖介导的,Notch、Osmosensory、Smoothened、Toll、Wnt等12条信号通路中与肝再生相关的上调基因数和下调基因数,分别为26和23、164和54、59和51、5和1、22和14、21和10、1和1、4和11、23和11、32和17。肝再生中基因上调表达次数和下调表达次数分别为188和128、464和190、308和207、13和5、88和46、123和50、2和1、20和43、148和30、174和62。结论除Osmosensory和脂多糖介导的信号通路在肝再生中作用较小,Smoothened信号通路作用减弱外,其他9条信号通路在肝再生中作用增强。
Objective To study the regulating effect of in the regulation of cell surface receptor linked signal transduction pathway-related genes on rat liver regeneration （LR） at transcriptional level. Methods Genes involved in the above pathways were obtained by data collection and literature review. The gene expression changes during LR were checked by Rat Genome 230 2.0 array, and LR-related genes were identified by comparing gene expression difference between partial hepatectomy （PH） and sham operation （SO） groups. Results 491 genes were identified as LR-related. There were 742 kinds of interactive relations in 226 before-mentioned genes. In the 12 kinds of signaling pathways cytokine and chemokine mediated, enzyme linked receptor, Gprotein coupled receptor, glutamate, antigen receptor-mediated, integrin-mediated, lipopolysaccharide-mediated, Notch, osmosensory, smoothened, Toll and Wnt the numbers of up-regulated and down-regulated LR-related genes were 26 and 23, 164 and 54, 59 and 51, 5 and 1, 22 and 14, 21 and 10, 1 and 1, 4 and 11, 23 and 11, 32 and 17 respectively. In LR, the numbers of up-regulated and the down-regulated genes were 188 and 128, 464 and 190, 308 and 207, 13 and 5, 88 and 46, 123 and 50, 2 and 1, 20 and 43, 148 and 30, 174 and 62 in the corresponding pathways. The genes which up regulated mainly occurred 0.5-16, 30, 42, 54, 96 hours and which down regulated mainly occurred 18-24, 36, 60-72, 120-168 hours. Conclusion In liver regeneration, lipopolysaccharide-mediated and osmosensory signaling pathway have less effect, and the smoothened signaling pathway＇s role is attenuated, but the activities of the other 9 pathways are enhanced.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第3期316-322,共7页
Acta Anatomica Sinica
基金
国家973计划前期研究专项基金资助(2006CB708506)
关键词
部分肝切除
细胞表面受体
信号通路
肝再生
基因组2302.0芯片
大鼠
Partial hepatectomy （PH）
Cell surface receptor
Linked signal transduction pathways
Liver regeneration： Genome 230 2.0 arra
Rat
