摘要
目的:探讨翻译控制肿瘤蛋白(TCTP)mRNA在肝再生中的表达情况和生物学意义.方法:对健康成年雄性SD大鼠进行2/3部分肝切除以建立肝再生模型,分别于肝切除术后不同时间点收集再生肝组织,在显微镜下进行有丝分裂指数评价,利用流式细胞术分析细胞周期分布变化;半定量RT-PCR法检测TCTP mRNA的表达.结果:肝细胞有丝分裂指数在术后3～12h明显升高.细胞周期分析显示,肝切除术后1h呈现G0/G1向S期迁移,G2/M期细胞比例在3h呈现升高,6h升高最为显著.TCTP mRNA的表达在肝切除术后1h时轻微上调,在3～12h呈显著升高,之后下降至初始水平.结论:在肝再生组织中TCTP mRNA表达呈动态变化,可能与有丝分裂和细胞增殖密切相关.
AIM： To investigate the expression of TCTP mRNA and its biological significance in liver regeneration. METHODS： Liver regeneration model was established by a two-thirds partial hepateetomy（PH） Jn healthy adult male SD rats. The regenerative liver tissues were harvested at different time-points after PH. The mitotic index（MI） was evaluated under microscope and the distribution in G0/G1-, S- and G2/M-pbase subpopulation was deter- mined by flow cytometry. The expression of TCTP mRNA was measured by semi-quantitative RT-PCR. RESULTS： After PH, the MI of hepatocytes increased significantly at 3 to 12 h. Cell cycle analysis showed a G0/G1- to S-phase transition at 1 h and an increased proportion of G2/M-phase cells at 3 h, followed by the most significant increase at 6 h. The mRNA expression of TCTP was up-regulated slightly at 1 h, markedly increased at 3 to 12 h and then declined to the original level. CONCLUSION： The dynamic changes of TCTP mRNA expression may be related to the mitosis and cell proliferation in liver regeneration.
出处
《第四军医大学学报》
北大核心
2009年第13期1192-1194,共3页
Journal of the Fourth Military Medical University
基金
河南省杰出青年科学基金(0512000800)
河南省高校杰出科研人才创新工程基金(2004kjcX017)
关键词
肝再生
翻译控制肿瘤蛋白
有丝分裂
细胞周期
liver regeneration
translationally controlled tumor protein
mitosis
cell cycle
