摘要
以持异性引物扩增了金焰笛鲷(Lutjamts fulviflamma)的核糖体第一转录间隔区(ITS-1),扩增产物经克隆后测序,测得 ITS-1长度为566 bp。其中 A、T、G、C 4种碱基的含量分别为14.1%、16.1%、30.2%、39.6%,G+C(69.8%)含量明显高于 A+T 含量(30.2%)。将此引物在笛鲷属其他4种鱼类中扩增,发现该对引物有很好的通用性;比较发现在不同种中 ITS-1存在着较大的差异,适合将其应用于分子系统学和种质资源方面的研究。
The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma, then the purified PCR productions were cloned into T-vector and sequenced by M13 ＋/-primers. As a result, 566 bp nucleotide sequences of rDNA-ITS1 were obtained. The average contents of A, T, G and C were 14. 1% , 16. 1% , 30. 2% and 39.6% , respectively, the contents of GC （69.8%） were obviously higher than those of AT. Using these primers, ITS-1 region can be amplified in other four Lutjan~ species, but the lengths of ITS-1 were largely different between different species.
出处
《南方水产》
CAS
2006年第5期61-64,共4页
South China Fisheries Science
基金
广东省重大科技兴海项目(A200099A01)
