摘要
采用聚合酶链式反应直接测序法,获得紫红笛鲷(Lutjanusargentimaculatus)、白星笛鲷(L.stellatus)、千年笛鲷(L.sebae)、勒氏笛鲷(L.russelli)、红鳍笛鲷(L.erythropterus)5种笛鲷属鱼的线粒体细胞色素b(Cytb)425bp序列,并结合基因库中的斜带笛鲷(L.decussatus)Cytb同源序列进行比较分析,共发现88个核苷酸位点存在变异(20.7%)。用MEGA2.1软件中的Pairwisedistance工具计算了6种笛鲷属鱼类的相对遗传距离,表明6种笛鲷属鱼类的序列差异在0.096—0.129之间,其中红鳍笛鲷和紫红笛鲷序列差异最大为0.129,千年笛鲷与红鳍笛鲷的序列差异最小为0.096;序列变异的转换/颠换比值较低,平均只有2.160。
The DNA sequences of 425 bp mitochondrial cytochrome b of Lutjanus argentimaculatus, L. stellatus,L. erythropterus, L. sebae, L. russelli and L. decussatus were analyzed. The result indicated that there were 88 nucleotide sites substituted ((20.7)%). 'Pairwise distance' of MEGA2.1 was used to compute the genetic distances among 6 species in Lutjanus. The substitutions (transition+transversion) among the six sequences ranged from 0.096 to 0.129, with an average of 0.109. The highest substitution occurred at L. argentimaculatus and L. erythropterus (0.129) and the lowest subsitution at L. erythropterus and L. sebae (0.096). The transition-transversion ratios of the sequence substitutions of 6 species in Lutjanus ranged from 1.095 to 3.594, with an average of 2.160.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2004年第4期87-92,共6页
Journal of Tropical Oceanography
基金
广东省科技兴海重大项目(A200099A01)
中国水产科学研究院人才基金项目
