摘要
以相应引物聚合酶链式反应(PCR)扩增长耳珠母贝(Pinctadachemnitzi)核核糖体RNA基因第2转录间隔区(ITS 2),PCR产物大小约500bp,经克隆、测序,所得序列包含5.8S和28SrRNA基因部分序列和ITS 2全序列,4种碱基含量分别为27.11%(A)、24.95%(G)、21.26%(T)和26.68%(C)。5个克隆的DNA序列差异为0.0%—0.4%,不存在个体内变异。对其潜在应用进行了讨论。
The ribosomal internal transcribed spacer 2 (ITS-2) of wild pearl oyster, Pinctada chemnitzi, was amplified via PCR using relevant primers. The size of PCR product was about 500bp DNA. The DNA products were cloned and sequenced. The sequence contained a partial sequence of 5.8S rRNA gene, a complete sequence of ITS-2 and a partial sequence of 28S rRNA gene. The contents of A, G, T and C in ITS-2 sequence were 27.11%, 24.95%, 21.26% and 26.68%, respectively. The alignment comparison indicated that the divergence of ITS-2 sequences form five clones of an individual was 0.0%-0.4%, showing no variability at individual level. The potential application of ITS-2 to phylogenetic research of pearl oysters was discussed, too.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2004年第5期81-84,共4页
Journal of Tropical Oceanography
基金
国家"863"计划项目(2002AA603022)
博士后基金资助项目
关键词
长耳珠母贝
ITS-2
序列分析
Pinctada chemnitzi
internal transcribed spacer 2 (ITS-2)
sequencing
