摘要
应用G-显带技术对来源于同一膀胱癌手术标本的三株新建的克隆性膀胱癌细胞系（BLZ211，BLX211和BLS211）的核型进行分析，发现所有的核型均为超二倍体，染色体众数分别是63、62和69；每个细胞都缺失一条9号染色体，7号染色体多为三条或四条；每个细胞系都有五个固定出现的异常染色体，其中四条是三个细胞系共有的：①4号染色体的结构重排，4pter→4q35：：？，②9号染色体臂间倒位，inv（9）（p21q22），③2个不能识别的标记染色体．M3和M4。本文研究结果表明9号和7号染色体改变是这三株人膀胱癌细胞的原发染色体畸变。
The karyotypic characteristics of three clonal human transitional cell carcinoma lines (BLZ211, BLX211 and LBS211 ) newly established from the same parts of surgically rernoved tumor were investigated with a G-banding method. All of the cells were hyperdiploid. The modal chromosomal numbers were found to be 63, 62 and 69 respectively. Monosomy of chromosome 9 was consistently present in all cells. Chromosome 7 was trisomic or tetrasomic in the cells. Five nonrandom chromosomal aberrations (Marker 1~5) were found in each karyotype. Four of them were observed in each cell line. These were ① a specific chromosomal rearrangement of chromosome 4, 4pter →4q35: ?, ② apericentric inversion of chromosome 9, inv (9)(p21 q22 ). ③ the component parts of marker chromosome M3 and M4 could not be identified. These findings indicate that involvement of chrornosome 9 and 7 may be the primary karyotypic changes in thethree transitional cell carcinoma lines.
出处
《西安医科大学学报》
CSCD
1997年第4期426-429,共4页
Journal of Xi'an Medical University(Chinese)
关键词
细胞系
染色体畸变
膀胱肿瘤
bladder transitional cell carcinorna
cell line
chrornosomal aberration
