摘要
目的通过对食管癌组织及癌旁组织中早期生长反应基因1（Egr-1）表达的研究，探讨Egr-1mRNA与食管癌患者预后的关系。方法应用RT—PER技术检测68例食管癌患者Egr—1mRNA表达情况，并对68例患者进行生存期随访，分析其与患者预后的关系。结果Egr—lmRNA在正常食管组织表达最高为1．201±0．347，在癌旁组织表达次之，为0．945±0．336，在癌灶中心组织表达最低，为0．56710．404（F=12．709，P〈0．01，P〈0、05）；68例癌组织和癌旁组织标本中Egr—lmRNA均阳性表达的21例，单一表达阳性的21例，均阴性表达的26例。随访68例术后患者，术后1年生存组60例、术后3年生存组35例；2组Egr-1mRNA均阳性表达率分别为30、00％和65．71％（P〈0．01）；2组阳性表达者生存率分别是94．44％和86．96％（P〉0．05）。结论从癌旁组织到癌组织Egr—lmRNA表达减弱可能与食管癌的癌变有一定关系；食管癌组织Egr—lmRNA表达水平与患者生存期有关系，对食管癌预后判断有重要参考价值。
Objective To study the expression of early growth response gene-1 （ Egr-1 ） in esophageal carcinoma tissue,and to explore the relationship between Egr-I and the survival time of the patients with esophageal carcinoma. Methods RT-PCR was performed to detect the expression of Egr-lmRNA in 68 cases of esophageal carcinoma. The relationship between survival time and prognosis was analyzed. Results The expression of Egr-lmRNA was the lowest （ 0.567 ± 0.404 ） , （ 0. 945 ± 0. 336 ） and （ 1. 201 ± 0.347 ） in esophageal carcinoma tissue, para-cancerous tissue and normal esophageal mucosa tissue, respectively （ F = 12. 709, P 〈 0.05, P 〈 0.00 ）. 21 cases showed the positive expression of Egr-lmRNA of both the esophageal carcinoma tissue and the para-cancerous tissue. 21 cases showed the positive expression of Egr-lmRNA in a single esophageal carcinoma tissue or the para-cancerous tissue. 26 cases showed the negative expression of Egr-1 mRNA both in the esophageal carcinoma tissue and the paracancerous tissue. The positive rate of Egr-lmRNA expression was 65.71% and 30.00% in the groups of the survival time for three years and the groups of the survival time for one year（ P 〈 0.05 ）. The survival rates in the two groups with positive expression of Egr-1 mRNA were 94.44% and 86.96%, respectively （ P 〉 0.05 ）. Conclusion Decreased level of EGR1 expression may be related to esophageal carcinogenesis. The expression level of Egr-lmRNA might be associated with the survival time of the patients with esophageal carcinoma. Egr-1 expression in esophageal carcinoma tissue may be of great value for determining prognosis of esophageal carcinoma.
出处
《中国综合临床》
北大核心
2008年第6期524-526,共3页
Clinical Medicine of China
基金
湖北省教育厅基金资助（2004D012）
关键词
食管癌
EGR—lmRNA
生存期
预后
Esophageal tumor
Early growth response gene-l mRNA
Survival time
Prognosis
