摘要
目的研究一氟磷酸谷酰胺及αD3对去势大鼠四肢骨的影响。方法3个月龄SD雌性大鼠39只,随机分成4组。假手术组(sham),去卵巢不处理组(OVX),去卵巢单用一氟磷酸谷酰胺组(MFP),一氟磷酸谷酰胺与αD3联合用药组(MFP+αD3)。动物去势后6周,MFP组给予一氟磷酸谷酰胺按0.45mgF-加13.56mgCa2+/(kg·d)溶于蒸馏水后灌胃。MFP+αD3组补充αD3按0.1μg/(kg·d)灌胃。每天1次,每周6次,连续12周。在HPIAS10000型图像分析系统进行骨组织形态测量,采用双能X线测量股骨胫骨骨密度。结果骨形态计量结果显示去势组股骨骨小梁面积、骨小梁宽度均低于假手术组,骨小梁间隔宽于假手术组(P<0.01),氟化物及αD3组骨量较去势组明显增加(P<0.01)接近假手术组水平;去势后股骨骨密度低于假手术组,药物治疗后骨密度升高。结论一氟磷酸谷酰胺组与去势组比能明显促进股骨骨量的增加及骨密度的提高。
[Objective] The objective of this study was to investigate the effects of Monofluorphosphate and αD3 on the femur of ovariectomy rats for clinical practice. [Methods] 39 female Sprague-Dawley rats about 90 days of age were randomly divided into the following treatment groups and treated for 12 weeks： Sham, Ovx and treated groups. Animals of treated groups were received Monofluorphosphate for 6 days/week at a dose of 0.45 mg F^-＋13.56 mg Ca2＋/kg·d body weight, by gastric feeding at the begininging of seven weeks after ovariectomy. They were also treated with αD3 at a dose of 0.1 μg/kg·d body weight. All rats were sacrifised by ether. The right femur was collected for histomorphometry and bone mineral density measurement. [Results] The bone area and bone thick in the OVX group were significantly lower than those in sham group. While the trabecular separation of the OVX group was significantly higher than that of sham groups. The mass of bone increased dramaticly in treated group. The bone mineral density of femur in OVX decreased after surgery compared with that of sham. Conversely it was increased in treated groups. [Conclusion] Monofluorphosphate can obviously increase the mass of femur and the bone mineral density of femur.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第10期1350-1352,共3页
China Journal of Modern Medicine
基金
湖南省发改委资助项目(2004517)
关键词
氟化物
骨密度
股骨
Monofluorphosphate
bone mineral density
femur
