摘要
目的：观察β-catenin在毛囊干细胞中的分布表达变化，探讨其与细胞增殖的关系。方法：体外分离培养毛囊干细胞，绘制细胞生长曲线，采用ICC方法观察β-catenin在不同培养时相毛囊干细胞中的定位表达变化。结果：培养2d便可见细胞生长，6d时细胞数达到最高峰；培养前3d β-catenin表达于胞浆及胞膜，第5—6天出现核内表达，伴有ki67核内表达；继续培养，β-catenin集中在胞核表达，胞浆呈弱表达，并表现与细胞增殖的相关性。结论：β-catenin核内转位是毛囊干细胞增殖的重要信号调节机制。
Objective：To investigate the translocated expression of β -catenin in hair follicle stem cells （HF- SCs） in vitro, and to further explore the relationship between β -catenin and cell proliferation. Methods： HFSCs were obtained from rat follicles by microdissection. Following the growth curve definited, the immunocytochemistry was used to detect the expression of β -catenin in different phases. Results： The growth curve showed that cells proliferated quickly at day 3 after inoculation, and the cell proliferation reached the peak at the day 6 and main- tained in the following days. Meanwhile, β - catenin was obviously expressed in cytoplasm and cell membrane of HFSCs in the third day. After 5 to 6 days of culture, β - catenin appeared in nuclear, accompanied with strong expression of ki67, a nuclear proliferative antigen. Then, β - catenin was translocated in nuclear completely instead of cytoplasm. Conclusion ： All the data displayed a correlation between β - catenin and cell proliferation, suggesting that β -catenin might be an essential regulative molecule involving in HFSC proliferation.
出处
《西北国防医学杂志》
CAS
2008年第3期180-182,共3页
Medical Journal of National Defending Forces in Northwest China
基金
国家自然基金资助项目(30671888)
