摘要
平菇原生质体制备最佳条件是菌丝培养5 d、酶浓度1.5%、pH 5.5、0.6 mol/L甘露醇渗透压稳定剂、酶解温度30℃、酶解时间2 h、OS培养基。原生质体过滤、纯化、稀释后涂布再生培养基,再生率为2.6%。
The optimized conditions of preparation and regeneration of Pleurotus erygii protoplast were mycelium cultured for 5 days, enzyme 1.5 % , pH5.5,0.6 mol/L mannitol, digest for 2 hours in 30 ℃ in OS medium. The protoplast preparation was filtered, purified, diluted, then spread on regeneration medium, the regeneration rate was 2.6%.
出处
《微生物学杂志》
CAS
CSCD
2008年第2期82-84,共3页
Journal of Microbiology
关键词
平菇
原生质体
制备
再生
Pleurotus erygii
protoplast
preparation
regeneration
