摘要
初步研究了以可溶形式在大肠杆菌中表达的重组人肿瘤坏死因子α（ｒｈＴＮＦα）衍生物１１ａ中试水平的发酵工艺，包括工程菌（ＤＨ５α／ｐＢＶＴＮＦΔ）密度、诱导时间及宿主菌对菌体收得率、目的蛋白表达量及其比活性的影响，建立了较稳定的中试发酵方法，以此条件５０Ｌ罐发酵的工程菌，１００００ｒ／ｍｉｎ离心３０ｍｉｎ菌体收得量可达湿重１４ｇ／Ｌ培养物；目的蛋白表达量约占菌体总蛋白的３１％；比活性约为１×１０８Ｕ／ｍｇ蛋白。
The aim of this study is to develop a fermentation procedure on Escherichia coli DH5α that expresses a soluable human TNFα derivative(rhTNFα D11a). The effect of bacteria density, induction time and different host strains on the collection ratio, percentage of the recombinant protein on the ratio were analysed. Stable fermentation parameters were obtained. 10000 rpm centrifugation for 30min could achieve 14g wet bacteria per liter in 50 L fermentum under the established condition. The TNF derivative was about 31% of total protein of the host. Its activity could reach 1×10 8U/mg protein.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1997年第5期341-343,共3页
Medical Journal of Chinese People's Liberation Army
关键词
肿瘤坏死因子
发酵
大肠杆菌
tumor necrosis factor
fermentation
Escherichia coli
