摘要
将核心抗原基因起始码下游的序列，构建两个ＣＡＴ报告基因表达质粒，即ｐＣＡＴＮⅠ和ｐＣＡＴＮⅡ，转染ＨｅｐＧ２与ＣＶ－１细胞后，均可使ＣＡＴ报告基因表达，表现出启动子活性。
Based on finding out a new RNA polymerase Ⅱ transcript within HBcAg gene of HBV adr NC-1 and the start point of the transcript located about 100bp downstream of initiate codon of HBcAg gene, two plasmid pCATN Ⅱ and pCATN Ⅰ were constructed by insertion of the HBV DNA fragments (210bp and 240bp respectively) into the pSVo CAT. The 5′ end of the fragments just started at ATG codon of the C gene, and the 3′ end varied. The gene expression of pCATN Ⅱ and pCATN Ⅰ were made by transfection of the DNA into HepG2 and CV-1 cells. The CAT activities which expressed by the plasmids in these cells were determined by CAT assay procedure with 14 C chloramphenicol as substrate. The results showed that both two fragments (N Ⅱ and N Ⅰ ) of HBV DNA performed promoter activities in modulation of the CAT gene expression either in HepG2 cells or in CV-1 cells. The promoter core site and the related gene will be discussed later.
出处
《病毒学报》
CAS
CSCD
北大核心
1997年第4期314-318,共5页
Chinese Journal of Virology
基金
自然科学基金
