摘要
<正> 核酸序列分析表明,乙型肝炎(乙肝)病毒核心抗原基因(C基因)编码区内存在两个ATG。目前认为第二个ATG为乙肝病毒C抗原的起始密码子,两个ATG之间的序列称为前C序列,共87bp。Uy、Rutter、Roossinck及景新等的研究表明,含前C序列时,C基因在大肠杆菌中的表达是形成具有HBeAg活性的P25~e膜蛋白,在哺乳动物细胞中的表达则是形成分泌性的HBeAg;不含前C序列时。
The chimeric plasmid pPC-5,P5-C26 were constructed by cloning individually the fragments of HBcAg gene with the entire pre-core sequence or with partial pre-core sequence ( 27bp before second ATG ) into the transfer vector pGJP-5 under the control of P7.5 promoter. The different C gene fragments were separately inserted into the region of vaccinia virus TK gene using homologous recombination technique in vivo. The TK-phenotype recombinant viruses, designated V-PC and V-C26 were assayed for the activities of HBeAg and HBcAg using ELISA method. Further studies indicated that the recombinant virus V-PC could secret only HBeAg into medium both in human TK--143 cells and chick embryo fibroblast cells and no HBcAg activity was found even in supernatants concentrated by 100 folds. Both HBeAg and HBcAg activities could be detected in cells lysates. The recombinant virus VMC-1 ( containing C gene with 12 bp before second ATG) and V-C26 could express both HBeAg and HBcAg activities in TK--143 cells, but not secreted into the medium. The above results implied that the entire pre-core sequence probably plays an important role in the secretion of HBeAg.
出处
《病毒学报》
CAS
CSCD
北大核心
1991年第2期170-175,共6页
Chinese Journal of Virology
基金
国家自然科学基金
关键词
乙肝病毒
核心抗原基因
痘苗
Hepatitis B virus core antigen gene Recombinantvaccinia Hepatitis B virus e antigea
