摘要
目的探讨系统性红斑狼疮（SLE）患者外周血Th极化状况的改变以及其受CD40／CD154和地塞米松（Dex）的影响。方法使用ELISA法检测SLE患者和对照组血浆IFN-γ和IL-10水平，分离、培养外周血单个核细胞（PBMC），应用CD40 mAb和Dex进行干预，使用ELISA法检测培养液上清IFN-γ和IL-10水平。结果SLE患者血浆IFN-γ水平明显低于对照组（P〈0．01，P〈0．05），活动期组血浆IL-10水平明显高于缓解期组和对照组（均P〈0．01）；CD40 mAb对PBMC培养液上清IFN-γ和IL-10水平无影响（均P〉0．05）；Dex可使SLE患者PBMC培养液上清IFN-γ水平升高（P〈0．05，P〈0．01），Dex可以明显降低SLE患者和对照组PBMC培养液上清IL-10水平（均P〈0．05）。结论SLE患者血浆IFN-γ水平降低、IL-10水平增高，存在Th2偏移；此种改变不受CD40／CD154影响但能被Dex抑制。
Objective To investigate the polarization of T helper in patients with systemic lupus erythmatosus （SLE） and its changing caused by CD40/CD154 and dexamethasone （Dex）. Methods The plasma concentrations of IFN-γ, and IL-10 were measured by ELISA. The cultured PBMCs from patients and healthy control were treated with CD40 mAb or Dex. The supernatant concentrations of IFN-γ, and IL-10 were measured by ELISA. Results The plasma concentration of IFN-γ, in SLE was lower than that in healthy control （P 〈 0.01, P 〈 0.05 ）. The plasma concentration of IL-10 in active SLE was higher than that in inactive SLE and healthy control （ all P 〈 0.01 ）. The supernatant concentrations of IFN-γ, and IL-10 were not affected by CD40 mAb. Dex could increase the supernatant concentration of IFN-γ, in SLE （P 〈0.05, P 〈0.01 ） and significant decrease IL-10 in three groups （ all P 〈 0.05）. Conclusion There were decreased plasma concentration of IFN-γ, and increased IL-10 and Th2 predominance in SLE. This predominance could not be affected by CD40/CD154 but could be affected Dex.
出处
《国际免疫学杂志》
CAS
2008年第1期1-4,共4页
International Journal of Immunology
基金
资金项目：广东省医学科学研究基金资助项目（A2001151）,广东省自然科学基金资助项目（04300354）
