摘要
应用高效液相色谱法测定人体血清中艾司唑仑和三唑仑的浓度。用反相Ｃ１８柱，甲醇—水—乙腈—四甲基乙二胺为流动相，冰醋酸调ｐＨ６．５，检测波长２５４ｎｍ，选用安定作内标，样品用无水乙醚在碱性条件下提取。血清中艾司唑仑和三唑仑的最低检测浓度分别为８．３５μｇ／Ｌ和２０μｇ／Ｌ，平均回收率分别为９２．６％和８９．４％，ＲＳＤ分别为３．２１％和５．５３％。本法简便、快速、灵敏度高，适用于艾司唑仑和三唑仑的治疗药物监测和中毒时的快速筛选。
A rapid specific and sensitive HPLC method for the determination of meclobemide in serum was developed.The mobile phase was methanol-water-TMEDA-glacial acetic acid(70∶30∶0 44∶0 35).Alkalinized sample was extracted with anhydrous ether.The organic phase was separated and evaporated to dryness under a stream of nitrogen.The residue was redissolved in the mobile phase and detected at 254nm.The linear range was from 10 to 2400μg/L,r=0 9994.The average recovery was 101 3%,the relative standard deviation was from 2 55% to 6 31%.The useful method for human pharmacokinetic studies was developed.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1997年第1期5-8,共4页
Chinese Journal of Pharmaceutical Analysis
