摘要
目的探讨粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）在哮喘嗜酸细胞（Ｅｏｓ）炎症中的作用。方法建立哮喘豚鼠实验模型，用雷公藤处理，观察肺组织Ｅｏｓ密度，斑点分子杂交检测支气管肺组织ＧＭ－ＣＳＦｍＲＮＡ含量。结果哮喘组肺组织Ｅｏｓ浸润密度增加，ＧＭ－ＣＳＦｍＲＮＡ表达水平增高，与对照组相比差异有显著性（Ｐ＜０．０５）。雷公藤处理后ｍＲＮＡ表达显著降低，但Ｅｏｓ浸润密度减少不明显。结论ＧＭ－ＣＳＦｍＲＮＡ表达增加可能是导致哮喘Ｅｏｓ气道炎症的原因。雷公藤能抑制ＧＭ－ＣＳＦｍＲＮＡ表达，可能具有哮喘抗炎治疗的潜在应用价值。
Objective To explore the role of granulocyte macrophage colony stimulating factor (GM CSF) in eosinophil inflammation of asthma airway. Methods Guinea pig models of asthma were established with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with injection of tripholide, density of eosinophil infilatration in airway tissues was observed under microscope and expression of GM CSF mRNA in airway tissues was detected with dot hybridization by Dig labeled cDNA probe. Results expression of GM CSF mRNA in asthmatic animals were higher markedly than that in the tripholide treated and the control groups. However, there was no statistically differences of density of eosinophils infiltration between the asthma and the tripholide treated groups. Conclusions GM CSF might participate in eosinophil inflammation in airway of asthmatic animals and tripholide might be of potential value in anti inflammation for asthma.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1997年第5期291-293,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金
关键词
哮喘
动物模型
mRNA
雷公藤
GM-CSF
Asthma
Granulocyte macrophage
Colony stimulating factor
Gene expression
Animals
Tripholide
