摘要
对熏衣草微体快速繁殖和壮苗技术进行了研究。适合于熏衣草试管苗繁殖的培养基为MS+6—BA2.0mg/L+NAA 0.05mg/L+蔗糖30g/L+琼脂7g/L,培养28d,繁殖系数达8以上;培养基中附加CCC质量浓度为0.05mg/L^0.5 mg/L时对熏衣草试管苗繁殖和壮苗有利;熏衣草试管苗生根用IAA的效果较好,适宜于生根的培养基配方为MS+IAA1.0mg/L+蔗糖20g/L+琼脂7g/L,培养25d生根率为91.6%;生根试管苗移栽成活率为73%。
Rapid micro-propagation and cultivating strong plantlets of Lavandula vera were studied. The result showed that the optimum medium for micro-propagation of plantlet of Lavandula vera was MS＋ 6-BA 2.0mg/L ＋ NAA 0.05mg/L ＋ sucrose 30mg/L ＋ agar.7g/L, the propagation coefficient is above 8 after 25 days. The medium MS ＋ CCC 0.5mg/L - 0.5 mg/L, is favorable to cultivate strong seedlings. The medium MS ＋ IAA 1.0mg/L ＋ sucrose 20mg/L ＋ agar 7g/L is better for rooting, the rooting rate is 91.6% after 25 days. The survival rate of the transplant is 73%.
出处
《中国草地学报》
CSCD
2008年第2期116-120,共5页
Chinese Journal of Grassland
基金
山东省农业良种工程项目计划(鲁科农字〔2006〕90号)
关键词
熏衣草
微体快速繁殖
植物生长调节剂
试管苗
壮苗培育
Lavandula vera
Rapid micro-propagation
Plant growth regulator
Plantlet in test tube
Cultivating strong plantlets
