摘要
目的研究苦参碱(Mat)对体外培养的Hela细胞增殖细胞核抗原(PCNA)表达及细胞周期的影响,为Mat治疗类风湿关节炎(RA)提供可能的实验室依据。方法用免疫组织化学法测定PCNA表达水平,用流式细胞术测定细胞周期变化,并与甲氨蝶呤(MTX)作比较。结果Mat、MTX均可使体外培养的Hela细胞的PCNA表达水平明显降低,还可以使细胞周期发生明显的变化,干预组Hela细胞停留在G1期的比例要比未干预组明显增加(P<0.05),而S、G2期的细胞比例比未干预组则明显减少(P<0.05)。结论苦参碱有下调Hela细胞PCNA表达水平,阻滞细胞周期的作用,且机制与MTX有相似之处。推测Mat有治疗RA的前景。
Objective To investigate the effects of Matrine （Mat） on PCNA expression and cell cycling in invitro cultured Henrietta Lacks strain of cancer cells （Hela cells） and compare these with methotrexate （MTX）, aiming at providing laboratory evidences for a therapeutic prospect in which Matrine is used for RA treatment. Methods The cell cycle was measured using flow cytometry （FCM）, and the level of proliferating cell nuclear antigen （PCNA） with immunohistochemistry （IHC）. The results were compared between Mat and MTX. Results Both Mat and MTX led to significantly suppressed expression of PCNA as well as ahered cell cycling in Hela cells. Compared with non -intervention group, more cells in the intervention group were trapped at G1 phase （P〈0.05） and fewer caught at S and G2 phases （P〈0.05）. Level of PCNA of Mat-treated and MTX-treated groups declined significantly compared with controls （P〈 0.05）, but these changes did not differ statistically between the two groups. Conclusion The findings suggested inhibited PCNA expression and retarded cell cycle in Hela cells treated with Matfine was similar to those with MTX. Mat was shown to offer promise for treatment of RA.
出处
《中国药物与临床》
CAS
2008年第3期180-183,共4页
Chinese Remedies & Clinics
关键词
苦参碱
甲氨蝶呤
HELA细胞
细胞增殖
细胞周期
Matrine
Methotrexate
Henrietta Lacks strain of cancer cells
Cell proliferation
Cell cycle
