摘要
目的:研究苦参碱(matrine,Mat)体外对兔晶状体上皮细胞细胞周期的影响。方法:将0.5,1.0和1.5g/L的苦参碱作用于体外培养的兔晶状体上皮细胞,通过流式细胞仪检测其细胞周期各时相的百分比。结果:不同浓度梯度的苦参碱作用体外培养的兔晶状体上皮细胞72h后,与对照组及各浓度的Mat组之间相互比较,随着苦参碱浓度增高,G0-G1期细胞数量明显增加(P<0.05),S期细胞明显减少(P<0.05),呈明显的剂量-效应关系。结论:苦参碱能明显抑制体外培养的兔晶状体上皮细胞的增殖活性和细胞有丝分裂。
AIM .To study the effects of matrine （Mat） on cell cycle of rabbit lens epithelial cells （RLECs） in vitro. METHODS-0.5g, 1.0g and 1.5g/L Mat was added to the RLECs in vitro for 72 hours culture respectively. The cell cycle of RLECs was analyzed by flow cytometer （FCM）. RESULTS. The percentage of RLECs in S stage was decreased significantly by Mat （ P 〈 0.05 ）, while with a significant increase in the percentage of G0-G1 stage cells （P〈0.05）. CONCLUSION Mat could effectively prevent cell division to inhibit the proliferation of RLECs in vitro.
出处
《国际眼科杂志》
CAS
2010年第2期233-234,共2页
International Eye Science
关键词
苦参碱
晶状体上皮细胞
细胞增生
后囊膜混浊
兔
细胞周期
matrine
lens epithelial cell cell proliferation
posterior capsular opacification
rabbit cell cycle
