摘要
目的观察保存液中加入丹参对低温保存小肠微循环的保护作用．方法杂种猪１２只，随机分成２组，对照组（ｎ＝６），供肠采用Ｅｕｒｏｃｏｌｉｎｓ（ＥＣ）液保存；丹参组（ｎ＝６），ＥＣ液中加入丹参注射液（２０ｍｇ／Ｌ），二组供肠均４℃保存２４ｈ后自体移植．观察移植小肠组织损伤和肠粘膜伊文思蓝渗出量（微循环通透性）．结果再灌注０５ｈ丹参组肠粘膜组织损伤评分低于对照组（３２０±０４２ｖｓ４６５±０５５，Ｐ＜００５），微循环通透性（μｇ／ｇ湿重）较低（１３６７５±０５４２ｖｓ２０４０６±０４５９，Ｐ＜００１）．结论丹参能有效地抑制低温保存小肠微循环通透性的增加，减轻血管内皮细胞损伤．
AIM To investigate the effect of Salvia miltiorrhiza (SM) on microcirculatory permeability of small bowel graft in a swine segmental small bowel autotransplantation model. METHODS Twelve outbred pigs were divided into two groups. In control group ( n =6), graft was preserved with Eurocollins (EC) solution; and in SM group ( n =6), graft was preserved with EC solution containing compound SM in jection (20ml/L). The graft was autotransplanted after 24 hour storage at 4℃.Scores of tissue injury and mucosal containment of Evan (microcirculatory permeability) were studied. RESULTS The recipient survival rates were 60% (control group) and 100% (SM group), respectively, at 30min after reperfusion, and scores of tissue injury (3 20±0 42 vs 4 65±0 55, P <0 05) and microcirculatory permeability (in μg/g wet weight) (13 675±0 542 vs 20 406±0 459, P <0 01) in graft were significantly lower in SM group than that in control group. CONCLUSION SM can attenuate endothelial cell injury of graft and reduce microcirculatory permeability.
关键词
丹参
药理学
小肠移植
微循环
salvia miltiorrhiza/pharmacology
intestine, small/transplantation
\ intestine, small/blood supply
microcirculation/drug effects
