摘要
作者研究了低温(4℃)保存小肠微循环通透性的变化。采用猪自体节段性小肠移植模型,实验分为三组。Ⅰ组:灌洗压力10.8kPa冷缺血时间58min;Ⅱ组:灌洗压力13.7kPa,冷缺血时间61min;Ⅲ组:灌洗压力10.8kPa,冷缺血时间为24h。移植小肠均采用Eurocollins液灌洗、保存。采用伊文思蓝比色法测定肠微循环通透性,移植术后0.5h移植小肠微循环通透性分别为正常的3.6倍、7倍和12倍,氧自由基生成增加。本研究表明低温保存小肠微循环通透性明显增加,影响因素包括灌洗压力、冷缺血时间和自由基等。 ·
This study investigated changes in microcirculatory permeability of small bowel grafts in pigs preserved by cold storage. White outbred pigs were divided into three groups. Group I, perfusion pressure was 10. 8 kPa(110cmH2O). cold ischemia time (CIT) was 58 min; group I . perfusion pressure was 13. 7 kPa (140 cmHjO) > CIT 61 mini group 1 , perfusion pressure was 10,8 kPa, CIT 24h. Grafts were perfused and preserved with 4'C EuroCollins solution, microcirculatory permeability was measured by Evan's blue colormetric method. The microcirculatory permeability of grafts postoperation 0. 5h increased by 3. 6 times ( group I), 7 times (group I ) and 12 times (group I ) compared with normal small intestines, respectively, products of oxygen free radical increased. The results confirmed that microcirculatory permeability of small bowel grafts increased significantly. Influential factors included perfusion pressure, cold ischemia time, and oxygen free radicals etc.
关键词
小肠移植
微循环通透性
小肠
低温保存
Microcirculatory permeability Small intestine transplantation Oxygen free radicals
