摘要
C57BL／6N小鼠尾静脉注射Lewis肺癌细胞后，腹腔注射脂质体干扰素α（L-IFN－α）和未包裹干扰素α（IF－α），连续10d；测小鼠肺湿重、肺转移给节数和ConA诱导的脾细胞DNA参入量。结果IFN－α5×104、20×104U／kg组，小鼠肺湿重较对照组减少14．1％、19．6％，肿瘤肺转移结节数减少32．7％、50.0％，ConA刺激的脾细胞DNA参入量增加24．6％、46．8％。1／10剂量的L-IFN－α可产生相同的药理活性。小鼠环磷酰胺50mg／kgip3d后，尾静脉注射瘤细胞，实验处理同上。结果L-IFN-α和IFN－α对环磷酰胺处理小鼠的Lewis肺癌细胞肺转移的抑制作用更为明显。本文结果表明，IFN－α可抑制小鼠Lewis肺癌肺转移，促进ConA刺激的脾细胞增殖，并与剂量明显相关。脂质体包裹IFN－α可使其生物活性提高约10倍。对免疫功能受抑小鼠作用更为显著。
C57BL/6N mice were administrated iv with Lewis lung carcinoma cells, and then ip interferon-α liposome (L-IFN-α) or interferon-α (IFN-α) for ten days. The lung weight, metastasis foci and proliferation of splenocytes in mice were observed.The results showed that IFN-α inhibited the lung metastasis of Lewis lung carcinoma and increased proliferation of splenocytes in mice. ip 1 ~20 ×104U/kg of IFN-α for 10d, lung weight of thece decreased by 3. 1 % ~ 18. 8 %, the numbers of metastasis foci decreased by 8. 2 %~49. 9 %, and [3H]TdR incorporation increased by 1. 8 % ~46.7 %. After envelopment of IFN-α by liposome, its potency of anti-lung-metastasis of Lewis lung carcinoma and promoting proliferation of splenocytes increaed 11 times as against unenveloped IFN-α. L-IFN-αhad a more powerful effects of anti-metastasis and increasing proliferation of splenocytes in the mice administrated previously with cyclophosphamide than in the unadministrated mice. These results indicated that IFN-α liposome is a prospective preparation.
出处
《中国生化药物杂志》
CAS
CSCD
1997年第4期174-177,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
脂质体
干扰素Α
肺癌
肿瘤转移
脾细胞
Liposome, Interferon-α,Lewis lung carcinoma, Neaplansma metastasis, Splenocytes
