摘要
目的建立人胃癌耐羟基喜树碱细胞株即SGC-7901/HCPT,并对其生物学特性进行研究。方法采用浓度梯度递增法建立SGC-7901/HCPT;用细胞计数法描绘SGC-7901/HCPT和亲代细胞株即SGC-7901的生长曲线和群体倍增时间;用四甲基偶氮唑盐(MTT)比色法检测SGC-7901/HCPT对多种常用抗肿瘤药物的耐药特性;用流式细胞仪检测SGC-7901/HCPT与SGC-7901的细胞周期分布。结果历时9个月建成人SGC-7901/HCPT;SGC-7901/HCPT增殖减慢,倍增时间比SGC-7901延长8.2h;SGC-7901/HCPT对HCPT的耐药指数为9.583,与丝裂霉素(MMC)、5-氟脲嘧啶(5-Fu)、顺铂(CDDP)、长春新碱(VCR)、阿霉素(ADM)、柔红霉素(DNR)、足叶乙苷(VP-16)均无交叉耐药;SGC-7901/HCPT的S期和G2～M期的细胞比例比SGC-7901增多。结论本课题建立了SGC-7901/HCPT,其生物学特性不同于SGC-7901,具有耐药细胞株的基本生物学特性。
Objective To establish the camptothecin（HCPT） -resistant stomach cancer cell line SGC-7901/HCPT with its characteristics studied. Methods Cell growth curve was painted and the doubling time was accouted by cell counting assay; the camptothecin-resistant stomach cancer cell line SGC-7901/HCPT was established by repeatedly exposing stomach cancer cell line SGC-7901 to increased gradually concentration of HCPT. The resistant index of the resistant cell line to different chemitherapeutic drugs was determined by the methyl thiazolyl tetrazolium（MTY） assay. Cell cycle distribution was measured by flow cytometry（FCM ）. Results The HCPT-resistant human stomach cancer cell llne SGC-7901/HCPT was established after 9 months. The rate of cell proliferation of SGC-7901/HCPT was longer than that of SGC-7901 SGC-7901/HCPT indicated drug-resistance to HCPT, the drug resistant index being 9.583, but were not cross-resistant to Mitomycin C（MMC）, Cisplatin （C DDP ）, 5 -fluorouracil （5 -Fu ）,Vincristine sulfate （ VCR ）,Adriamycin （ADM）, Daunorubicin （DVR）, Etoposide （VP- 16 ）. The number of cells in S phrase and G2～M phrase increased（P〈 0.005 ） ,suggesting that the DNA cynthesis was increased in SGC-7901/HCPT. Conclusion The camptothecin-resistant stomach cancer cell line SGC-7901/HCPT is established. It can be used for further experiments.
出处
《中国现代医生》
2008年第3期15-17,共3页
China Modern Doctor
基金
四川省科技厅基金资助课题(02SC02-077)
关键词
人胃癌细胞株
羟基喜树碱
耐药
Human stomach cancer cell line
Camptothecin
Drug resistance
