摘要
采用体外造血细胞培养技术，应用高温、利多卡因、足叶乙苷（足叶乙甙，ＶＰ－１６）联合法，研究其在体外对白血病细胞的杀伤作用。结果表明：高温、利多卡因、ＶＰ－１６三者联用比高温＋利多卡因、高温＋ＶＰ－１６，能更有效地杀伤白血病细胞，而正常粒单系克隆形成单位（ＧＭ－ＣＦＵ）仍约有５０％存活。４１℃６０ｍｉｎ＋０．７５ｍｍｏｌ／Ｌ利多卡因＋８．５μｍｏｌ／ＬＶＰ－１６联合处理后，ＨＬ－６０、Ｕ９３７、Ｋ５６２细胞分别只存活０．０１％、９．４０％、３３．６６％。另外，从这个联合方案说明对于不同类型的白血病效果不一，在其自身骨髓移植（ＡＢＭＴ）时，体外净化骨髓要采用不同的净化方案。
sing the semi- solid t-CFU and GM-CFU culture method ,we investigated the effect of hyperthermia , lidocaine , etoposide on leukemic cell lines. and normal human bone marrow GM-CFU colony growth in vitro. The results showed that the combination of hyperthermia , lidocaine and etoposide could produce more additive tumor cell killing than hyperthermia plus lidocaine or , hyperthermia plus etoposide, while , under the same experimental conditions , normal hematopoietic precursors were spared , following hyperthermia 41 ℃ for 60min plus 0. 75mmol/L lidocaine plus 8. 5umol/L etoposide treatment ,the survival rate was 0. 01% for HL-60,9.94% for U937, 33. 97% for K562. These results also showed that the treatment had various effect on the different kinds of leukemia , so the different purging treatment should be used in ABMT.
出处
《中山医科大学学报》
CSCD
1994年第3期189-193,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
白血病
体外净化
利多卡因
ANLL
purging in vitro
lidocaine
etoposide
heat treatment
