摘要
目的:探讨人胃癌耐羟基喜树碱细胞株即SGC-7901/HCPT的耐药机制。方法:通过光镜和电镜观测SGC-7901/HCPT与人胃癌细胞株即SGC-7901形态和超微结构差异和免疫细胞化学的方法检测SGC-7901/HCPT与SGC-7901之间的TOPO-Ⅰ、TOPO-Ⅱ、P-gp、GST-π、BCL-2表达异同来探讨SGC-7901/HCPT的可能耐药机制。结果:(1)光镜下SGC-7901/HCPT细胞大小不一致,形态不规则,胞浆较丰富,核小而不规则,可见巨核现象;而SGC-7901细胞呈圆梭形,大小一致,核圆形;电镜下SGC-7901/HCPT表面指状突起明显,胞浆内有较多的核糖体及粗面内质网,线粒体较多。(2)SGC-7901/HCPT的TOPO-Ⅰ表达较SGC-7901明显增加,差异具有显著性;而TOPO-Ⅱ、P-gp、GST-π、BCL-2的表达差异无显著性。结论:SGC-7901/HCPT趋向细胞成熟分化,这可能是其产生耐药的形态学表现。TOPO-Ⅰ的表达下降则可能是SGC-7901/HCPT耐HCPT形成的分子基础。
Objective： To investigate drug- resistance mechanism of the camptothecin （HCPT）-resistant stomach cancer cell line SGC-7901/HCPT. Methods： The ceil image and its ultra micro-structure of parental and resistant ceils were observed with the optical microscope and electron microscope. Expression of TOPO- Ⅰ , TOPO- Ⅱ α, P-gp, GST-π and BCL-2 of parental and resistant cells were assessed with S-P immunocytochemical Method. Results： The size of the SGC-7901/HCPT cells were different, with irregular shape and more abundant cytoplasm, small and irregular nucleus, many of them with a lobulate nucleus under the optical microscope. The SGC-7901 has similar size and regular shape, with fewer cytoplasm, regular and large nucleolus. Ribosome, rough endoplasmic reticulums and mitochondria in SGC-7901/HCPT increased under electron microscope. The expression level of TOPO- Ⅰ in SGC-7901/HCPT showed a significant reduction than that of the parent ceil line（ P 〈 0. 05 ）. The staining of TOPO-Ⅱ α, P-gp, GST-π and BCL-2 are no significant different between them. Conclusion： Our results suggest that ceil differentiation toward the maturity and down regulated expression of TOPO-Ⅰ are correlated to the drug resistance of the camptothecin-resistant stomach cancer ceil line.
出处
《四川肿瘤防治》
2007年第1期10-12,共3页
Sichuan Journal of Cancer Control
基金
四川省科技厅基金资助课题(02SC02-077)
关键词
人胃癌细胞株
羟基喜树碱
耐药
Human Stomach Cancer Cell Line
Camptothecin
Drag Resistance
