摘要
目的:研究生地对原代分离的大鼠肺间质成纤维细胞(fibrob last,Fb)Ⅰ、Ⅲ型胶原(ColⅠ、ColⅢ)表达的影响。方法:生地经提取分离后得到不同部位的提取物。大鼠肺间质成纤维细胞经原代分离传代后,加入不同浓度的生地提取物及生地注射液作用72 h,采用RT-PCR的方法测定其中Ⅰ、Ⅲ型胶原的量。结果:水提醇沉上清液中以低聚糖为主的部分除个别浓度外,其余浓度对ColⅠ表达均有抑制作用,高浓度对ColⅢ表达有一定的抑制作用;醇沉沉淀部分2×10-2g/mL的浓度对ColⅠ表达抑制作用明显,各浓度对ColⅢ表达均有抑制作用;生地注射液各浓度均对ColⅠ的表达均有抑制作用,低浓度(5×10-3g/mL)对ColⅠ、ColⅢ表达均有明显抑制作用。结论:生地中某些成分能够抑制大鼠肺间质成纤维细胞ColⅠ、ColⅢ的表达,是生地对肺纤维化起治疗作用的机制之一。
AIM： To investigate the effeet of Rehmannia glutinosa Liboseh on collagen Ⅰ and Ⅲ of in pulmonary interstitial fibroblast in rat. METHODS： The eomponents of Radix Rehmanniae were extraeted and isolated to several parts. Primary pulmonary fibroblasts were separated from lung interstitial tissue of rat. After being repro- dueed for several generations, fibroblasts were eultured in the extraets of Radix Rehmanniae and Shengdi Solution for injeetion in several eoneentrations respeetively for 72 hours, and then eontents of Col Ⅰ and Col Ⅲ of these fibroblast were determined by RT-PCR. RESULTS： The part eontaining mainly oligosaeeharide from preparation that deeoeted with water and deposited with aleohol eould inhibit Col Ⅰ with a few exeeptions eoneentration while it could inhibit Col Ⅲ at high concentration. The part of deposit inhibited Col I obviously in the concentration of 2 - 10-2 g/mL and eould inhibit Col Ⅲ in all the eoneentrations. Shengdi Solution for injeetion eould inhibit Col Ⅲ in all the eoneentrations. CONCLUSION： Some eonstitutents of Radix Rehmanniae earl inhibit the expression of Col Ⅰ and Col Ⅲ of pulmonary fibroblast which is one of mechanisms that Radix Rehmanniae takes effect on fibrosis disease in pulmonary interstitial tissue.
出处
《中成药》
CAS
CSCD
北大核心
2008年第2期175-178,共4页
Chinese Traditional Patent Medicine
基金
上海市科技攻关课题,编号:05DZ19102
关键词
生地
纤维化
Ⅰ型胶原
Ⅲ型胶原
Radix Rehmanniae
oligosaccharid
collagen Ⅰ
collagen Ⅲ
