摘要
[目的]运用多种技术手段研究香加皮水提取物诱导人食管癌细胞TE-13凋亡。[方法]利用MTT染色在普通光镜下观察细胞凋亡形态学变化并观测药物对肿瘤细胞的抑制率;通过流式细胞仪分析人食管癌TE-13细胞凋亡率、细胞周期变化;DNA琼脂糖凝胶电泳方法检测肿瘤细胞凋亡的DNA水平变化;激光扫描共聚焦显微镜(LSCM)检测细胞内钙离子浓度变化。[结果]经香加皮水提取物处理的人食管癌细胞TE-13出现核固缩、凋亡小体、新月状浓染区等细胞凋亡形态学改变,各剂量药物处理组的肿瘤细胞均表现生长抑制,且具有剂量依赖性,250μg/ml香加皮水提取物处理组的抑制率达91.02%。DNA琼脂糖凝胶电泳呈现典型的梯形电泳条带。药物处理组的肿瘤细胞更多地被阻止于G2/M期,各剂量组均出现明显的凋亡变化,最大凋亡率可达20.3%。各剂量药物处理组的肿瘤细胞内的Ca2+浓度明显高于对照组(P<0.01)。[结论]香加皮水提取物可明显抑制人食管癌细胞TE-13的生长增殖,诱导细胞凋亡,并能改变该肿瘤细胞周期分布,阻止细胞周期于G2/M期。
[Objective] To study the induetive effect of the extract of Chinese silkvine mot-bark on apoptosis of human esophageal cancer cells TE-13. [Methods] The cell morphology of apoptosis and inhihitory rate of the cancer cell were analyzed by MTT staining and a ordinary microscope. The apoptosis rate of TE-13 cells and cell cycles were studied by the flow ey, tometer. Changes of apoptosis at the DNA level were studied by agarose gel electrophoresis; changes of the intracellular Ca^2＋ concentration were analyzed by the laser scanning confocal microscope （LSCM）. [ Results] After treatment with the extract of Chinese silkvine root-bark, TE-13 cells showed morphologie features of apoplosis such as pyknosis, apoptotic body, thick stained area as crescents.Treated cancer cells were inhibited and dose dependent and maximal inhibition rate was 91.02%. DNA agarose gel eleetrophoresis showed characteristic DNA ladder. After treatment with the extract of Chinese silk,,ine root-bark, most TE-13 cells were arrested in the G2/M phase and appeared apoptosis, maximal apoptosis rate of TE-13 was 20.3%. Intracellular Ca^2＋ concentration in the experimental groups was obviously higher than that in the controls （P 〈 0.01）. [ Conclusion] The extract of Chinese silkvine root-bark can obviously inhibit human esophageal cancer cells TE-13 to proliferate, induce the apoptosis and arrest the cell cycle in the C2,/M phase.
出处
《现代预防医学》
CAS
北大核心
2008年第5期942-943,945,共3页
Modern Preventive Medicine
关键词
香加皮
人食管癌细胞TE-13
细胞凋亡
Chinese silkvine root-bark
Human esophageal cancer cells TE-13
Apoptosis
