摘要
将4种贝母的甲醇提取物经薄层色谱法粗分离后,直接用双波长紫外分光光度法测定其含量。结果表明,该方法线性关系好,腺苷和胸苷标准曲线的相关系数均为09999,同时也发现平贝、炉贝和伊贝中腺苷都占核苷总量60%以上,而浙贝中仅占约40%。
A convenient method for the quantitative analysis of adenosine and thymidine in Fritillaria bulbs was developed by means of dual wavelength ultraviolet spectrophotometry. This method has good linear relationship and the interrelated coefficient of standard curve for adenosine and thymidine were all found to be 0 9999. The methanol extracts of four species of Fritillaria have been analyzed with this method. The results show that the the bulbs of four species contain different quantities of adenosine and thymidine, which indicates that the nucleosides may be responsible for the anti coagulation activity of Fritillaria.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第8期617-619,共3页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
国家教委留学回国人员科研资助
关键词
贝母
腺苷
胸苷
双波长
紫外分光光度法
Fritillaria
Adenosine
Thymidine
Dual wavelength ultraviolet spectrometry