摘要
目的:建立HPLC测定天南星、半夏、白附子中8种核苷成分含量的方法。方法:采用Phenomenex Luna PFP(2)柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相,梯度洗脱,流速0.8 mL.min-1,柱温35℃,检测波长260 nm。结果:尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、肌苷、鸟苷、胸苷、腺苷进样量分别在0.004 34～0.434μg(r=0.999 9),0.002 34～0.234μg(r=0.999 9),0.004 07～0.407μg(r=0.999 7),0.010 90～1.090μg(r=0.999 9),0.002 01～0.201μg(r=0.999 9),0.00732～0.732μg(r=0.999 9),0.001 22～0.122μg(r=0.999 7),0.002 07～0.207μg(r=0.999 9)有良好的线性关系;平均回收率分别为104.2%(RSD 2.1%),98.7%(RSD 2.4%),103.7%(RSD 3.0%),99.4%(RSD 1.4%),101.8%(RSD 2.0%),102.9%(RSD 1.9%),98.3%(RSD 2.8%),102.3%(RSD 2.7%)。结论:方法简便、准确、快速,结果可靠,为测定天南星、半夏、白附子中8种核苷成分提供依据。
Objective： To establish an HPLC method for simultaneous determination of eight nucleosides in Rhizoma Arisaema, Rhizoma Pinellie and Rhizoma Typhonii. Method： The chromatographic separation was achieved on a Phenomenex Luna PFP （2） （4.6 mm × 250 mm, 5 μm） column with methanol-water solution as mobile phase （ gradient elution）. The flow rate was 0.8 mL -min-I, the column temperature was maintained at 35 ℃ and the detective wavelength was set at 260 nm. Result： The linear ranges of uracil, hypoxanthine, xanthine, uridine, inosine, guanosine, thymidine and adenosine were 0. 004 34-0. 434 μg （r =0. 999 9）, 0. 002 34- 0.234 μg （r =0.999 9）, 0.004 07-0.407 μg （r =0.999 7）, 0.010 90-1.090 μg （r =0.999 9）, 0.002 01- 0.201 μg （r=0.999 9）, 0.007 32-0.732μg （r =0.999 9）, 0.001 22-0. 122 μg （r =0.999 7）, 0.002 07- 0.207 μg （ r = 0. 999 9 ） respectively. The average recoveries of the eight nucleosides were 104.2% , 98.7% , 103.7%, 99.4%, 101.8%, 102.9%, 98.3%, 102.3% and RSDs were 2.1%, 2.4%, 3.0%, 1.4%, 2.0%, 1.9%, 2.8%, 2.7% respectively. Conclusion： The method is simple, accurate and rapid. It can be used for determination of nucleosides in Rhizoma Arisaema, Rhizoma Pinellie and Rhizoma Typhonii.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第16期59-62,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划(973计划)项目(2009CB522804)
关键词
天南星
半夏
白附子
核苷
高效液相色谱
Rhizoma Arisaema
Rhizoma Pinellie
Rhizoma Typhonii
nucleoside
HPLC
