摘要
为了探讨人胎胰腺ＩＡＰＰ－与Ｉｎｓ－ＩＲ细胞的个体发生及其分布与共存，应用免疫组织化学ＰＡＰ法，对２２例１１～３０周人胎胰内ＩＡＰＰ－和Ｉｎｓ－ＩＲ细胞进行定位研究。结果显示，１１周人胎儿胰内已可见到ＩＡＰＰ－ＩＲ细胞单个散在，Ｉｎｓ－ＩＲ细胞较多，未见ＩＡＰＰ与Ｉｎｓ共存的细胞。１６周以后，ＩＡＰＰ－ＩＲ细胞逐渐增多，或散在分布，或三五成群。与邻片比较观察，可见到ＩＡＰＰ与Ｉｎｓ定位的细胞相应，但ＩＡＰＰ－ＩＲ细胞数量仍较Ｉｎｓ－ＩＲ细胞少，且免疫染色较弱。至３０周，在邻片同一胰岛内可见较多ＩＡＰＰ和Ｉｎｓ定位相同的细胞。研究结果提示，在胎儿期胰岛细胞已开始合成ＩＡＰＰ，并与Ｉｎｓ共存于Ｂ细胞，但ＩＡＰＰ与Ｉｎｓ两者在表达时序上有差异。本文还对ＩＡＰＰ与Ｉｎｓ在胎儿胰腺内共存的意义进行了讨论。
In this study,the islet amyloid polypeptide(IAPP) immunoreactivity(IR)cells of 22 human fetal pancreas from 11 to 30 weeks of gestation were investigated with immunohistochemical PAP method.The ontogeny of IAPP IR cells and the coexistence of IAPP with insulin in pancreas were explored.The results showed that,at week 11 of fetal age,the islets differed in size with fewer scattered IAPP IR cells and more insulin IR cells.From week 16 of gestation.the number of IAPP IR cells gradually increased.At the same time,it was also found that the location of IAPP IR cells was partly in agreement with that of insulin IR cells.When compared with insulin IR cells in the same one islet,IAPP IR cells appeared fewer and their immunostaining was weaker.At week 30 of gestation,the more coexisting cells for IAPP and insulin were found.The results indicate that during the human fetus,IAPP were synthesized and coexisted with insulin in B cells,but both IAPP and insulin were different in their gene express order.The significance about the coexistence of IAPP with insulin in fetal pancreas was disscused in this paper. [WT5HZ〗
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第3期329-332,共4页
Acta Anatomica Sinica
关键词
胰岛淀粉样多肽
胰岛素
免疫组织化学
胎儿
胰腺
Islet amyloid polypeptide
Insulin
Coexistence
Immunohistochemistry
Human fetus
