摘要
用免疫组织化学双重染色法,在一张切片上同时显示胰岛PP细胞与A细胞。观察生后发育期间大鼠全胰PP细胞、A细胞和PP-Glu共存细胞的形态、分布和数量。结果表明,PP细胞突起可伸达A细胞,PP和Glu在胞质内共存呈分散或分区两种形式,胰岛可分为PP细胞型、A细胞型和PP-A细胞型,PP细胞和PP-Glu共存细胞的计数表明,在生后7天最低,28天最高。
PP cells and A cells were simultaneously displayed on one and the same slice with a double-imm-unohistochemical technique. Observation was made on morphology, distribution and prportion of PP cells, A cells and coexis-tant PP-Glucagon cells of rat pancreas during the development after birth. PP cells were seen to giveoff long cytoplasm processes closely contacting with A cells; Coexistant PP-Glucagon cells showed twoforms, the two substances were dispersed or seperated in the cytoplasm. There were three types of islets,PP cells type, A cells type and PP-A cells type; PP cells and PP-Glucagon cells showed a minimum in7-day group and maximum in 28-day group.
出处
《北京医科大学学报》
CSCD
1990年第6期410-412,482,共3页
Journal of Peking University(Health Sciences)
关键词
胰岛
胰多肽细胞
免疫组织化学
Immunohistochemistry
Islet PP cells
Islet A cells