摘要
目的明确ESBLs在郑州地区的基因型分布。方法收集78株临床分离产ESBLs革兰阴性杆菌,混合后提取总质粒作为模版,用特异性引物进行PCR扩增。结果显示TEM、SHV、OXA1、OXA20、CTX-M1、CTX-M14和CTX-M25的泳道有阳性条带。结论郑州地区目前已有TEM、SHV、OXA和CTX-M四种亚型的ESBLs流行。
Objective To investigate the genotype characteristics of extended - spectrum J3 -laetemases （ESBLs） in Zhengzhou. Methods Total 78 strains of ESBLs producing isolates of germs with confirmed phenotype were used. PCR were performed by using specific primers for TEM, SHV,OXA and CTX - M. Results TEM, SHV, CTX - M1, CTX - Ml4, CTX - M25, OXA1 OXA20＇ s lanes are positive. Conclusion TEM ,SHV,OXA and CTX - M are the prevalent genotype of ESBLs in Zhengzhou.
出处
《医药论坛杂志》
2008年第2期7-9,12,共4页
Journal of Medical Forum
基金
河南省医学科技攻关项目(200703071)
