摘要
检测鸡大肠杆菌超广谱β-内酰胺酶(ESBLs)的基因型并了解辽宁省鸡源性大肠杆菌产ESBLs基因型分布。对大连4个地区不同鸡场临床分离32株产ESBLs鸡大肠杆菌分别用TEM、OXA、CTX-M和SHV等4种通用引物进行PCR扩增确定其基因型。结果显示,32株鸡大肠杆菌均检出TEM型基因、12株检出CTX-M型基因、2株检出OXA型、未检出SHV型;TEM+CTX-M双阳性检出11株、TEM+OXA双阳性检出2株。TEM基因亚型占检出比例最高(100%)。目前辽宁省产ESBLs鸡大肠杆菌基因亚型以TEM为主,其次为CTX-M型,未发现SHV型;以TEM+CTX-M双阳性检出率最高(34.4%)。
To elucidate the genotype distributions of ESBLs produced by E. coli isolated from the fowl in Liaoning Province. Thirty-two ESBL-producing Escherichia coli were collected from four different chicken farms in Dalian, and were analyzed by PCR amplifying using the primers of TEM, OXA, SHV and CTX-M, respectively. The results showed that： thirty-two strains with TEM-type, twelve CTX-M-types and two OXA-types were detected except SHV-type was not found; moreover, eleven strains with multiple TEM ＋ CTX-M and two strains with TEM＋OXA genotypes were detected. The predominant genotype of ESBLs was TEM （100% detection rate） in chicken farms of Liaoning Province, and then were CTX-M, SHV was not found; the multiple TEM＋CTX-M positive rate reached up to 34.4%.
出处
《中国农学通报》
CSCD
北大核心
2011年第20期76-78,共3页
Chinese Agricultural Science Bulletin
基金
辽宁省教育厅高等学校科学研究项目"PRRSV辽宁分离株遗传变异分析及分子诊断方法研究"(L2010265)
