摘要
目的初步探讨金葡液以纤维蛋白胶为载体修复骨缺损的可行性。方法选用Wistar大鼠30只在颅顶骨两侧对称各制造直径为5 mm的圆形骨缺损模型后分为实验组、对照组及空白组,将纤维蛋白胶-金葡液复合物植入骨缺损处作为实验组,单纯纤维蛋白胶植入作为对照组,空白组不植入任何物质。于术后4,6周行大体观察,X线拍片,组织学检查,修复骨灰重与湿重测定。结果术后4,6周,X线片显示实验组缺损区的成骨面积百分比明显大于对照组及空白组,三组两两比较差异均有统计学意义(P<0.01);术后6周,组织学检查,实验组骨缺损内可见纤维蛋白胶已完全吸收,缺损已骨性愈合,对照组缺损内纤维蛋白胶已降解吸收,缺损边缘有大量新生骨质,空白组缺损边缘亦可见到少量新生骨质,其他部分为致密纤维结缔组织覆盖;修复骨湿重/灰重比值低于对照组与空白组,三组比值两两比较差异均有统计学意义(P<0.01)。结论金葡液以纤维蛋白胶为载体修复骨缺损是可行的,能减少金葡液局部注射的缺陷,提高其药物疗效。
Objective To study the feasibility of fibrin sealant as a carrier of staphylococcus aureus injection for repairing bone defects. Methods＂ Two 5 mm-diameter bone defects were manufactured symmetrically on both sides of the cranial bone in 30 Wistar rats. Then the rats were divided into experimental group, control group and blank group. Rats were implanted with complex of fibrin sealant and staphylococcus aureus injection in bone defect in experimental group, with fibrin sealant in control group, while nothing was implanted in blank group. After operation for 4 and 6 weeks, general observation, X-ray film and histological examination were performed,and osteotylus ashes weight and wet weight were determined. Results After operation for 4 and 6 weeks,X-ray revealed that percentage of new bone forming area in experimental group was higher than in control group and blank group, and there was statistically significant difference between three groups（ P 〈 0.01 ）. After 6 weeks, histological examination revealed that fibrin sealant had been completely absorbed in experimental group and bone defect completely healed. But in control group, fibrin sealant had been completely degraded and there were a large number of new bone at the edge of defect. A small amount of new bone could be seen at the edge of defect in blank group and other parts of defect had been covered by the dense fibrous connective tissue. The ratio of wet weight and ash weight of osteotylns in experimental group was lower than in control group and blank group, and there was significant difference between three groups（P〈0.01）. Conclusion Fibrin sealant as a carrier of staphylococcus aureus injection for repairing bone is feasible and can reduce the flaws of local injection of staphylococcus aureus injection and improve its drug efficiency.
出处
《山西医科大学学报》
CAS
2008年第2期129-132,共4页
Journal of Shanxi Medical University
关键词
纤维蛋白组织黏着剂
金葡液
骨缺损
修复
fibrin tissue adhesive
staphylococcus aureus injection
skull defect
repair
