摘要
目的：建立高效液相色谱-蒸发光散射检测法测定贞芪扶正颗粒中黄芪甲苷含量的方法。方法：色谱柱为Hypersil ODS2C，8（200mm×4．6mm，5μm），流动相为乙腈-水（35：65），流速为1mL·min^-1，载气为氮气（N2），气体压力为1．5MPa，雾化温度为35℃，气化温度为50℃。结果：黄芪甲苷进样量在2．38～51．00μg范围内的对数值与峰面积对数值呈良好线性关系；平均回收率为100．71％，RSD=1．94％（n=6）。结论：本方法迅速、准确、重现性好，可用于贞芪扶正颗粒的质量控制。
OBJECTIVE： To determine the content of Astragoloside IV in Zhenqifuzheng granule by HPLC- ELSD. METHODS： The samples were separated on Hypersil ODS2 C18（200 mm × 4.6 mm, 5 μm） with mobile phase consisted of acetonitrile - water （35 ： 65） at a flow rate of 1 mL · min^-1.The carrier gas using was N2 with a gas pressure of 0.15 MPa .The atomization temperature was 35 ℃ and the gasification temperature was 50 ℃ .RESULTS： This method showed a good linear relationship in the range of 2.38～51.00 μg for Astragaloside Ⅳ . The average recovery was 100.71% with RSD being 1.94% （ n = 6）. CONCLUSION ： The method is rapid, accurate and reproducible, and it can be used for the quality control of Zhenqi- fuzheng granule.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第6期444-446,共3页
China Pharmacy
